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验证数据展示

  • WB analysis of HT-1080 using 20660-1-AP

    WB analysis of HT-1080 using 20660-1-AP

    HT-1080 cells were subjected to SDS PAGE followed by western blot with 20660-1-AP (UBF1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

UBF1 Polyclonal antibody

UBF1 Polyclonal Antibody for WB, ELISA
Cat No. 20660-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, ELISA

别名

UBTF, UBF-1, UBF 1, UBF, NOR 90

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHT-1080 cells, HepG2 cells, MCF-7 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20660-1-AP targets UBF1 in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 upstream binding transcription factor, RNA polymerase I
别名 UBTF, UBF-1, UBF 1, UBF, NOR 90
计算分子量 89 kDa
观测分子量 85-90 kDa
GenBank蛋白编号NM_014233
基因名称 UBF1
Gene ID (NCBI) 7343
RRIDAB_2878718
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP17480
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

UBTF, also named as NOR-90 or UBF-1, is a 764 amino acid protein, which may form Homodimer. UBTF recognizes the ribosomal RNA gene promoter and activates transcription mediated by RNA polymerase I through cooperative interactions with the transcription factor SL1/TIF-IB complex. It binds specifically to the upstream control element. UBTF exists two isoform with MV 89 kDa and 85 kDa. (PMID: 1940801)

实验方案

Product Specific Protocols
WB protocol for UBF1 antibody 20660-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols