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验证数据展示

  • IP experiment of HeLa using 11564-1-AP

    IP experiment of HeLa using 11564-1-AP

    IP result of anti-BMSC UbP (IP:11564-1-AP, 4ug; Detection:11564-1-AP 1:1000) with HeLa cells lysate 2000ug.

  • IHC staining of human gliomas using 11564-1-AP

    IHC staining of human gliomas using 11564-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas using 11564-1-AP (BMSC UbP antibody) at dilution of 1:50 (under 10x lens).

  • WB analysis of mouse testis using 11564-1-AP

    WB analysis of mouse testis using 11564-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 11564-1-AP (BMSC UbP antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 11564-1-AP

    IF Staining of HepG2 using 11564-1-AP

    Immunofluorescent analysis of HepG2 cells using 11564-1-AP (BMSC UbP antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

BMSC UbP Polyclonal antibody

BMSC UbP Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 11564-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, ELISA

别名

Ubiquitin-like protein SB132, Ubiquitin-like protein 7, SB132, Bone marrow stromal cell ubiquitin-like protein, BMSC-UbP

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse testis tissue, HeLa cells, HepG2 cells, Jurkat cells, rat testis tissue
Positive IP detected inHeLa cells
Positive IHC detected inhuman gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11564-1-AP targets BMSC UbP in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 BMSC UbP fusion protein Ag2130 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ubiquitin-like 7 (bone marrow stromal cell-derived)
别名 Ubiquitin-like protein SB132, Ubiquitin-like protein 7, SB132, Bone marrow stromal cell ubiquitin-like protein, BMSC-UbP
计算分子量 380 aa, 41 kDa
观测分子量 41 kDa
GenBank蛋白编号BC030055
基因名称 UBL7
Gene ID (NCBI) 84993
RRIDAB_2212080
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96S82
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Ubiquitin is an important cellular signal that targets proteins for degradation or regulates their functions. UBL7 (ORF name SB132) encodes ubiquitin-like protein7 also named bone marrow stromal cell ubiquitin-like protein (BMSC-UbP) capable of binding ubiquitin. BMSC-UbP protein is derived from bone marrow stromal cells containing a ubiquitin-associated (UBA) domain at the C terminus that has been implicated in linking cellular processes and the ubiquitin system. BMSC-UbP mRNA is widely expressed in human multiple tissues and various tumor cell lines and is decreased in BMSC stimulated with phorbol myristate acetate (PMA) suggesting a role in regulation of BMSC function.

实验方案

Product Specific Protocols
WB protocol for BMSC UbP antibody 11564-1-APDownload protocol
IHC protocol for BMSC UbP antibody 11564-1-APDownload protocol
IF protocol for BMSC UbP antibody 11564-1-APDownload protocol
IP protocol for BMSC UbP antibody 11564-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols