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验证数据展示

  • WB analysis of K-562 using 12099-1-AP

    WB analysis of K-562 using 12099-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 12099-1-AP (UBLCP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 12099-1-AP

    IF Staining of HeLa using 12099-1-AP

    Immunofluorescent analysis of Hela cells, using UBLCP1 antibody 12099-1-AP at 1:50 dilution and FITC-labeled donkey anti-rabbit IgG (green). Blue pseudocolor = DAPI (fluorescent DNA dye).

  • IP experiment of K-562 using 12099-1-AP

    IP experiment of K-562 using 12099-1-AP

    IP result of anti-UBLCP1 (IP:12099-1-AP, 3ug; Detection:12099-1-AP 1:500) with K-562 cells lysate 1760ug.

  • IHC staining of human ovary cancer using 12099-1-AP

    IHC staining of human ovary cancer using 12099-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 12099-1-AP (UBLCP1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

UBLCP1 Polyclonal antibody

UBLCP1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 12099-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, ELISA

别名

UBLCP 1, Ubiquitin-like domain-containing CTD phosphatase 1, Nuclear proteasome inhibitor UBLCP1, EC:3.1.3.16, CPUB1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inK-562 cells, human brain tissue
Positive IP detected inK-562 cells
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHela cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12099-1-AP targets UBLCP1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 UBLCP1 fusion protein Ag2743 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ubiquitin-like domain containing CTD phosphatase 1
别名 UBLCP 1, Ubiquitin-like domain-containing CTD phosphatase 1, Nuclear proteasome inhibitor UBLCP1, EC:3.1.3.16, CPUB1
计算分子量 318 aa, 37 kDa
观测分子量 37 kDa
GenBank蛋白编号BC013425
基因名称 UBLCP1
Gene ID (NCBI) 134510
RRIDAB_2256901
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8WVY7
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for UBLCP1 antibody 12099-1-APDownload protocol
IHC protocol for UBLCP1 antibody 12099-1-APDownload protocol
IF protocol for UBLCP1 antibody 12099-1-APDownload protocol
IP protocol for UBLCP1 antibody 12099-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols