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UBR4 Polyclonal antibody, PBS Only

UBR4 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 15966-1-PBS

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Synonyms

验证数据展示

  • IHC staining of human pancreas cancer using 15966-1-AP (same clone as 15966-1-PBS)

    IHC staining of human pancreas cancer using 15966-1-AP (same clone as 15966-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 15966-1-AP (UBR4 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15966-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with UBR4 antibody included
  • IHC staining of human pancreas cancer using 15966-1-AP (same clone as 15966-1-PBS)

    IHC staining of human pancreas cancer using 15966-1-AP (same clone as 15966-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 15966-1-AP (UBR4 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15966-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with UBR4 antibody included
  • WB analysis using 15966-1-AP (same clone as 15966-1-PBS)

    WB analysis using 15966-1-AP (same clone as 15966-1-PBS)

    Various lysates were subjected to Tris-acetate gel system followed by western blot with 15966-1-AP (UBR4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15966-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 15966-1-AP (same clone as 15966-1-PBS)

    IF Staining of HeLa using 15966-1-AP (same clone as 15966-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using UBR4 antibody (15966-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 15966-1-PBS in a different storage buffer formulation.

  • IP experiment of HeLa using 15966-1-AP (same clone as 15966-1-PBS)

    IP experiment of HeLa using 15966-1-AP (same clone as 15966-1-PBS)

    IP result of anti-UBR4 (IP:15966-1-AP, 4ug; Detection:15966-1-AP 1:2000) with HeLa cells lysate 1760 ug. This data was developed using the same antibody clone with 15966-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy UBR4 Ready-To-Use IHC Kit. UBR4 primary antibody included.

产品信息

15966-1-PBS targets UBR4 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
Tested Reactivity human, mouse
Immunogen UBR4 fusion protein Ag8740 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ubiquitin protein ligase E3 component n-recognin 4
Synonyms
Calculated Molecular Weight 5183 aa, 570 kDa
Observed Molecular Weight570 kDa, 270 kDa
GenBank Accession NumberBC018694
Gene Symbol UBR4
Gene ID (NCBI) 23352
RRIDAB_2878202
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ5T4S7
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

UBR4 (ubiquitin N-recognin domain-containing E3 ligase 4), also named P600, is an E3 ligase that can bind N-end rule substrates. UBR4-deficient mice die during embryogenesis and exhibit pleiotropic abnormalities (PMID: 23431188). UBR4 plays a role in membrane morphogenesis and extracellular matrix-mediated cell survival and signaling, human papillomavirus (HPV) E7-mediated cellular immortalization, and migration and development of neurons (PMID: 33658508, 25084275). The full-length UBR4 protein has a predicted size of ~570 kDa.