验证数据展示
经过测试的应用
| Positive IHC detected in | mouse testis tissue, human testis tissue, human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | MCF-7 cells |
Planning an IHC experiment? We recommend our IHCeasy UBR5 Ready-To-Use IHC Kit. UBR5 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
22782-1-AP targets UBR5 in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, CoIP |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse |
| 免疫原 | UBR5 fusion protein Ag18753 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | ubiquitin protein ligase E3 component n-recognin 5 |
| 别名 | EDD1, EDD, EC:2.3.2.26, E3 ubiquitin-protein ligase, HECT domain-containing 1, E3 ubiquitin-protein ligase UBR5 |
| 计算分子量 | 2799 aa, 309 kDa |
| 观测分子量 | 309 kDa |
| GenBank蛋白编号 | BC137234 |
| 基因名称 | UBR5 |
| Gene ID (NCBI) | 51366 |
| RRID | AB_2879166 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O95071 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for UBR5 antibody 22782-1-AP | Download protocol |
| IF protocol for UBR5 antibody 22782-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun UBR5 promotes antiviral immunity by disengaging the transcriptional brake on RIG-I like receptors | ||
J Gastroenterol Hepatol C2orf40 inhibits hepatocellular carcinoma through interaction with UBR5. | ||
J Virol UBR5 Acts as an Antiviral Host Factor against MERS-CoV via Promoting Ubiquitination and Degradation of ORF4b | ||
Sci China Life Sci SUB1 promotes colorectal cancer metastasis by activating NF-κB signaling via UBR5-mediated ubiquitination of UBXN1 | ||
J Transl Med Inhibition of the E3 ligase UBR5 stabilizes TERT and protects vascular organoids from oxidative stress |
