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UBXN1 Polyclonal antibody

UBXN1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, ELISA and More (3)

Conjugate

Unconjugated

Publications

Cat no : 16135-1-AP

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Synonyms

2B28, LOC51035, SAKS1, SAPK substrate protein 1, UBA/UBX 33.3 kDa protein, UBX domain protein 1, UBXD10, UBXN1

验证数据展示

WB analysis using 16135-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 16135-1-AP (UBXN1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.
IHC staining of mouse testis using 16135-1-AP
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 16135-1-AP (UBXN1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with UBXN1 antibody included
IHC staining of mouse testis using 16135-1-AP
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 16135-1-AP (UBXN1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with UBXN1 antibody included

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Cat No. 16135-1-AP

Size

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经过测试的应用

Positive WB detected in	HeLa cells, human liver tissue, mouse cerebellum tissue, human brain tissue, human heart tissue, HepG2 cells, Ramos cells, SH-SY5Y cells, mouse liver tissue, rat liver tissue
Positive IHC detected in	mouse testis tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Planning an IHC experiment? We recommend our IHCeasy UBXN1 Ready-To-Use IHC Kit. UBXN1 primary antibody included.

Application	Dilution
Western Blot (WB)	WB : 1:5000-1:30000
Immunohistochemistry (IHC)	IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

KD/KO	See 3 publications below
WB	See 12 publications below
IHC	See 2 publications below
IF	See 2 publications below
IP	See 2 publications below
CoIP	See 2 publications below

产品信息

16135-1-AP targets UBXN1 in WB, IP, IF, IHC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications	WB, IHC, ELISA Application Description
Cited Applications	WB, IP, IF, IHC, CoIP
Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, pig
Immunogen	UBXN1 fusion protein Ag9146 种属同源性预测
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Full Name	UBX domain protein 1
Synonyms	2B28, LOC51035, SAKS1, SAPK substrate protein 1, UBA/UBX 33.3 kDa protein, UBX domain protein 1, UBXD10, UBXN1
Calculated Molecular Weight	33 kDa
Observed Molecular Weight	40-45 kDa
GenBank Accession Number	BC000902
Gene Symbol	UBXN1
Gene ID (NCBI)	51035
RRID	AB_2210480
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q04323
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

实验方案

Product Specific Protocols
WB protocol for UBXN1 antibody 16135-1-AP	Download protocol
IHC protocol for UBXN1 antibody 16135-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

发表文章

Species	Application	Title
human	WB	EMBO J A weakened interface in the P182L variant of HSP27 associated with severe Charcot-Marie-Tooth neuropathy causes aberrant binding to interacting proteins. Authors - T Reid Alderson View Article
human	WB	Cell Rep p53 promotes antiviral innate immunity by driving hexosamine metabolism Authors - Wenjun Xia View Article
human	WB,IP	EMBO Rep UBXN1 maintains ER proteostasis and represses UPR activation by modulating translation Authors - Brittany A Ahlstedt View Article
human	WB	Cancer Lett The CRISPR/Cas9 system targeting EGFR exon 17 abrogates NF-κB activation via epigenetic modulation of UBXN1 in EGFRwt/vIII glioma cells. Authors - Kai Huang View Article
human,mouse	WB,IHC	Front Immunol PTRF/Cavin-1 as a Novel RNA-Binding Protein Expedites the NF-κB/PD-L1 Axis by Stabilizing lncRNA NEAT1, Contributing to Tumorigenesis and Immune Evasion in Glioblastoma. Authors - Kaikai Yi View Article
human	WB	Sci Rep PMID: 29769705 Authors - Shotaro Uchida View Article

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UBXN1 Polyclonal antibody

UBXN1 Polyclonal Antibody for WB, IHC, ELISA

验证数据展示

WB analysis using 16135-1-AP

IHC staining of mouse testis using 16135-1-AP

IHC staining of mouse testis using 16135-1-AP

经过测试的应用

推荐稀释比

发表文章中的应用

产品信息

实验方案

发表文章

A weakened interface in the P182L variant of HSP27 associated with severe Charcot-Marie-Tooth neuropathy causes aberrant binding to interacting proteins.

p53 promotes antiviral innate immunity by driving hexosamine metabolism

UBXN1 maintains ER proteostasis and represses UPR activation by modulating translation

The CRISPR/Cas9 system targeting EGFR exon 17 abrogates NF-κB activation via epigenetic modulation of UBXN1 in EGFRwt/vIII glioma cells.

PTRF/Cavin-1 as a Novel RNA-Binding Protein Expedites the NF-κB/PD-L1 Axis by Stabilizing lncRNA NEAT1, Contributing to Tumorigenesis and Immune Evasion in Glioblastoma.

PMID: 29769705