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UBXN2B Polyclonal antibody

UBXN2B Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 25141-1-AP

Print datasheet

Synonyms

UBX domain-containing protein 2B, p97 cofactor p37, NSFL1 cofactor p37

验证数据展示

  • WB analysis using 25141-1-AP

    WB analysis using 25141-1-AP

    Neuro-2a cells lysates were subjected to SDS PAGE followed by western blot with 25141-1-AP (UBXN2B antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary cancer using 25141-1-AP

    IHC staining of human ovary cancer using 25141-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 25141-1-AP (UBXN2B antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of Neuro-2a using 25141-1-AP

    IF Staining of Neuro-2a using 25141-1-AP

    Immunofluorescent analysis of (4% PFA) fixed Neuro-2a cells using UBXN2B antibody (25141-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

经过测试的应用

Positive WB detected inNeuro-2a cells
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inNeuro-2a cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:400-1:1600
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25141-1-AP targets UBXN2B in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen UBXN2B fusion protein Ag18259 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name UBX domain protein 2B
Synonyms UBX domain-containing protein 2B, p97 cofactor p37, NSFL1 cofactor p37
Calculated Molecular Weight 331 aa, 37 kDa
Observed Molecular Weight37 kDa
GenBank Accession NumberBC113645
Gene Symbol UBXN2B
Gene ID (NCBI) 137886
RRIDAB_3085772
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ14CS0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

UBXN2B, also called p37, is a cofactor of the p97 AAA ATPase. UBXN2B regulates spindle orientation during mitosis by limiting the levels of cortical NuMA (PMID: 29222185).

实验方案

Product Specific Protocols
WB protocol for UBXN2B antibody 25141-1-APDownload protocol
IHC protocol for UBXN2B antibody 25141-1-APDownload protocol
IF protocol for UBXN2B antibody 25141-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols