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验证数据展示

  • WB analysis using 25141-1-AP

    WB analysis using 25141-1-AP

    Neuro-2a cells lysates were subjected to SDS PAGE followed by western blot with 25141-1-AP (UBXN2B antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary cancer using 25141-1-AP

    IHC staining of human ovary cancer using 25141-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 25141-1-AP (UBXN2B antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of Neuro-2a using 25141-1-AP

    IF Staining of Neuro-2a using 25141-1-AP

    Immunofluorescent analysis of (4% PFA) fixed Neuro-2a cells using UBXN2B antibody (25141-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

UBXN2B Polyclonal antibody

UBXN2B Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 25141-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, ELISA

别名

UBX domain-containing protein 2B, p97 cofactor p37, NSFL1 cofactor p37

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inNeuro-2a cells
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inNeuro-2a cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:400-1:1600
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25141-1-AP targets UBXN2B in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原 UBXN2B fusion protein Ag18259 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 UBX domain protein 2B
别名 UBX domain-containing protein 2B, p97 cofactor p37, NSFL1 cofactor p37
计算分子量 331 aa, 37 kDa
观测分子量37 kDa
GenBank蛋白编号BC113645
基因名称 UBXN2B
Gene ID (NCBI) 137886
RRIDAB_3085772
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ14CS0
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

UBXN2B, also called p37, is a cofactor of the p97 AAA ATPase. UBXN2B regulates spindle orientation during mitosis by limiting the levels of cortical NuMA (PMID: 29222185).

实验方案

Product Specific Protocols
WB protocol for UBXN2B antibody 25141-1-APDownload protocol
IHC protocol for UBXN2B antibody 25141-1-APDownload protocol
IF protocol for UBXN2B antibody 25141-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols