• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

UCH-L1/PGP9.5 Recombinant antibody, PBS Only

UCH-L1/PGP9.5 Uni-rAbTM Recombinant Antibody for WB, IHC, IF-P, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241107B2

Cat no : 84154-4-PBS

Print datasheet

Synonyms

PGP9.5, UCHL1, UCHL1/PGP9.5, PGP 9.5, PARK5

验证数据展示

  • WB analysis using 84154-4-RR (same clone as 84154-4-PBS)

    WB analysis using 84154-4-RR (same clone as 84154-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84154-4-RR (UCHL1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84154-4-PBS in a different storage buffer formulation.

  • IF Staining of mouse brain using 84154-4-RR (same clone as 84154-4-PBS)

    IF Staining of mouse brain using 84154-4-RR (same clone as 84154-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using UCH-L1/PGP9.5 antibody (84154-4-RR, Clone: 241107B2 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84154-4-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 84154-4-RR (same clone as 84154-4-PBS)

    IHC staining of mouse brain using 84154-4-RR (same clone as 84154-4-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84154-4-RR (UCH-L1/PGP9.5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with . This data was developed using the same antibody clone with 84154-4-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with UCHL1 antibody included
  • Affinity and Kinetic Characterization of 84154-4-RR

    Affinity and Kinetic Characterization of 84154-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84154-4-RR against Human UCHL1/PGP9.5 were performed. The affinity constant is below 1 pM.

Planning an IHC experiment? We recommend our IHCeasy UCHL1 Ready-To-Use IHC Kit. UCHL1 primary antibody included.

产品信息

84154-4-PBS targets UCH-L1/PGP9.5 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF-P, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen UCH-L1/PGP9.5 fusion protein Ag6490 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase)
Synonyms PGP9.5, UCHL1, UCHL1/PGP9.5, PGP 9.5, PARK5
Calculated Molecular Weight 25 kDa
Observed Molecular Weight27 kDa
GenBank Accession NumberBC000332
Gene Symbol UCHL1
Gene ID (NCBI) 7345
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP09936
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

UCHL1(Ubiquitin carboxyl-terminal hydrolase isozyme L1) is a member of a gene family whose products hydrolyze small C-terminal adducts of ubiquitin to generate the ubiquitin monomer. Expression of UCHL1 is highly specific to neurons and to cells of the diffuse neuroendocrine system and their tumors. It is present in all neurons(PMID: 6343558). This protein is present in brain at concentrations at least 50 times greater than in other organs and is a major protein component of neuronal cytoplasm (PMID:7217993). And UCHL1 is a Parkinson's disease susceptibility gene (PMID:15048890).