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验证数据展示

  • WB analysis using 66230-1-Ig (same clone as 66230-1-PBS)

    WB analysis using 66230-1-Ig (same clone as 66230-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66230-1-Ig (UCHL1 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 66230-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse cerebellum using 66230-1-Ig (same clone as 66230-1-PBS)

    IHC staining of mouse cerebellum using 66230-1-Ig (same clone as 66230-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 66230-1-Ig (UCHL1/PGP9.5 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66230-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with UCHL1 antibody included
  • FC experiment of Y79 using 66230-1-Ig (same clone as 66230-1-PBS)

    FC experiment of Y79 using 66230-1-Ig (same clone as 66230-1-PBS)

    1x10^6 Y79 cells were intracellularly stained with 0.4 ug UCHL1/PGP9.5 Monoclonal antibody (66230-1-Ig, Clone:1C9E11) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66230-1-PBS in a different storage buffer formulation.

  • IF Staining of SH-SY5Y using 66230-1-Ig (same clone as 66230-1-PBS)

    IF Staining of SH-SY5Y using 66230-1-Ig (same clone as 66230-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using UCHL1/PGP9.5 antibody (66230-1-Ig, Clone: 1C9E11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66230-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

UCH-L1/PGP9.5 Monoclonal antibody, PBS Only

UCH-L1/PGP9.5 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA
Cat No. 66230-1-PBS

产品说明书

CloneNo. 1C9E11

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, pig

应用

WB, IHC, IF/ICC, FC (Intra), ELISA

别名

PGP9.5, UCHL1, UCHL1/PGP9.5, PGP 9.5, PARK5

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy UCHL1 Ready-To-Use IHC Kit. UCHL1 primary antibody included.

产品信息

66230-1-PBS targets UCH-L1/PGP9.5 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human, mouse, rat, pig
免疫原 UCH-L1/PGP9.5 fusion protein Ag6547 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase)
别名 PGP9.5, UCHL1, UCHL1/PGP9.5, PGP 9.5, PARK5
计算分子量 25 kDa
观测分子量 27 kDa
GenBank蛋白编号BC000332
基因名称 UCHL1
Gene ID (NCBI) 7345
RRIDAB_2881621
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP09936
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Ubiquitin C-terminal hydrolase L1 (UCHL1) was originally identified as a neuronal protein that accounts for nearly 2% of total brain proteins. UCHL1 activity protects neurons from hypoxic injury, and binding of stroke-induced reactive lipid species to the cysteine 152 (C152) of UCHL1 unfolds the protein and disrupts its function. Reduced hydrolytic activity of mutant UCHL1 is implicated in the pathophysiologic process of Parkinson's and Alzheimer's disease due to abnormal neurotoxic protein aggregation. (PMID: 31356902, PMID: 30760601)