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  • Featured Product
  • KD/KO Validated

UCH-L1/PGP9.5 Monoclonal antibody, PBS Only

UCH-L1/PGP9.5 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

1C9E11

Cat no : 66230-1-PBS

Print datasheet

Synonyms

PGP9.5, UCHL1, UCHL1/PGP9.5, PGP 9.5, PARK5

验证数据展示

  • WB analysis using 66230-1-Ig (same clone as 66230-1-PBS)

    WB analysis using 66230-1-Ig (same clone as 66230-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66230-1-Ig (UCHL1 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 66230-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse cerebellum using 66230-1-Ig (same clone as 66230-1-PBS)

    IHC staining of mouse cerebellum using 66230-1-Ig (same clone as 66230-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 66230-1-Ig (UCHL1/PGP9.5 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66230-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with UCHL1 antibody included
  • FC experiment of Y79 using 66230-1-Ig (same clone as 66230-1-PBS)

    FC experiment of Y79 using 66230-1-Ig (same clone as 66230-1-PBS)

    1x10^6 Y79 cells were intracellularly stained with 0.4 ug UCHL1/PGP9.5 Monoclonal antibody (66230-1-Ig, Clone:1C9E11) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66230-1-PBS in a different storage buffer formulation.

  • IF Staining of SH-SY5Y using 66230-1-Ig (same clone as 66230-1-PBS)

    IF Staining of SH-SY5Y using 66230-1-Ig (same clone as 66230-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using UCHL1/PGP9.5 antibody (66230-1-Ig, Clone: 1C9E11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66230-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy UCHL1 Ready-To-Use IHC Kit. UCHL1 primary antibody included.

产品信息

66230-1-PBS targets UCH-L1/PGP9.5 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), ELISA Application Description
Tested Reactivity human, mouse, rat, pig
Immunogen UCH-L1/PGP9.5 fusion protein Ag6547 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase)
Synonyms PGP9.5, UCHL1, UCHL1/PGP9.5, PGP 9.5, PARK5
Calculated Molecular Weight 25 kDa
Observed Molecular Weight 27 kDa
GenBank Accession NumberBC000332
Gene Symbol UCHL1
Gene ID (NCBI) 7345
RRIDAB_2881621
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP09936
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Ubiquitin C-terminal hydrolase L1 (UCHL1) was originally identified as a neuronal protein that accounts for nearly 2% of total brain proteins. UCHL1 activity protects neurons from hypoxic injury, and binding of stroke-induced reactive lipid species to the cysteine 152 (C152) of UCHL1 unfolds the protein and disrupts its function. Reduced hydrolytic activity of mutant UCHL1 is implicated in the pathophysiologic process of Parkinson's and Alzheimer's disease due to abnormal neurotoxic protein aggregation. (PMID: 31356902, PMID: 30760601)