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  • Featured Product
  • KD/KO Validated

UCHL5 Polyclonal antibody, PBS Only

UCHL5 Polyclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 11527-1-PBS

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Synonyms

验证数据展示

  • WB analysis of mouse colon using 11527-1-AP (same clone as 11527-1-PBS)

    WB analysis of mouse colon using 11527-1-AP (same clone as 11527-1-PBS)

    mouse colon tissue were subjected to SDS PAGE followed by western blot with 11527-1-AP (UCHL5 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11527-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse kidney using 11527-1-AP (same clone as 11527-1-PBS)

    IHC staining of mouse kidney using 11527-1-AP (same clone as 11527-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 11527-1-AP (UCHL5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11527-1-PBS in a different storage buffer formulation.

产品信息

11527-1-PBS targets UCHL5 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen UCHL5 fusion protein Ag2095 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ubiquitin carboxyl-terminal hydrolase L5
Synonyms
Calculated Molecular Weight 326 aa, 37 kDa
Observed Molecular Weight 38 kDa
GenBank Accession NumberBC025369
Gene Symbol UCHL5
Gene ID (NCBI) 51377
RRIDAB_2877774
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y5K5
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

UCHL5 (Ubiquitin carboxyl-terminal hydrolase isozyme L5) is also named as UCH37 and belongs to the peptidase C12 family. It is functionally linked with multiple protein complexes and signal transduction pathways. UCH37's weight average molecular weight increases as a function of its concentration. By size-exclusion chromatography, the apparent molecular weight of UCH37 was 51, 56, 70, and 109 kDa at 0.15, 0.69, 2.4, and 9.5 mg/mL, respectively. Given UCH37's monomeric molecular weight of 37 kDa and its apparent elongated shape, its tendency toward an apparent molecular weight between 40 and 50 kDa at decreasing concentrations is appropriate for UCH37 monomers (PMID:21953935).