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验证数据展示

  • WB analysis using 68535-1-Ig (same clone as 68535-1-PBS)

    WB analysis using 68535-1-Ig (same clone as 68535-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68535-1-Ig (UCHL5 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68535-1-PBS in a different storage buffer formulation.

  • WB analysis of HCT 116 using 68535-1-Ig (same clone as 68535-1-PBS)

    WB analysis of HCT 116 using 68535-1-Ig (same clone as 68535-1-PBS)

    WB result of UCHL5 antibody (68535-1-Ig; 1:40000; incubated at room temperature for 1.5 hours) with sh-Control and sh-UCHL5 transfected HCT 116 cells. This data was developed using the same antibody clone with 68535-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

UCHL5 Monoclonal antibody, PBS Only

UCHL5 Monoclonal Antibody for WB, Indirect ELISA
Cat No. 68535-1-PBS

产品说明书

CloneNo. 2B8G11

宿主/亚型

Mouse / IgG2a

种属反应性

Human

应用

WB, Indirect ELISA

别名

CGI 70, INO80R, Ubiquitin thioesterase L5, UCH L5, UCH37, UCHL5

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

68535-1-PBS targets UCHL5 in WB, Indirect ELISA applications and shows reactivity with Human samples.

经测试应用 WB, Indirect ELISA Application Description
经测试反应性 Human
免疫原 fusion protein 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 ubiquitin carboxyl-terminal hydrolase L5
别名 CGI 70, INO80R, Ubiquitin thioesterase L5, UCH L5, UCH37, UCHL5
计算分子量 326 aa, 37 kDa
观测分子量 38 kDa
GenBank蛋白编号BC025369
基因名称 UCHL5
Gene ID (NCBI) 51377
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9Y5K5
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

UCHL5 (Ubiquitin carboxyl-terminal hydrolase isozyme L5) is also named as UCH37 and belongs to the peptidase C12 family. It is functionally linked with multiple protein complexes and signal transduction pathways. UCH37's weight average molecular weight increases as a function of its concentration. By size-exclusion chromatography, the apparent molecular weight of UCH37 was 51, 56, 70, and 109 kDa at 0.15, 0.69, 2.4, and 9.5 mg/mL, respectively. Given UCH37's monomeric molecular weight of 37 kDa and its apparent elongated shape, its tendency toward an apparent molecular weight between 40 and 50 kDa at decreasing concentrations is appropriate for UCH37 monomers (PMID:21953935).