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UCHL5IP Polyclonal antibody

UCHL5IP Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 17830-1-AP

Print datasheet

Synonyms

UIP1, UCHL5-interacting protein, UCHL5 interacting protein, HAUS7, HAUS augmin-like complex subunit 7

验证数据展示

  • WB analysis of MCF-7 using 17830-1-AP

    WB analysis of MCF-7 using 17830-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 17830-1-AP (UCHL5IP Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary using 17830-1-AP

    IHC staining of human ovary using 17830-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tissue slide using 17830-1-AP (UCHL5IP Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human ovary using 17830-1-AP

    IHC staining of human ovary using 17830-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tissue slide using 17830-1-AP (UCHL5IP Antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of MCF-7 using 17830-1-AP

    IF Staining of MCF-7 using 17830-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed MCF-7 cells using 17830-1-AP (UCHL5IP antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inMCF-7 cells
Positive IHC detected inhuman ovary tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

17830-1-AP targets UCHL5IP in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivityhuman
Immunogen UCHL5IP fusion protein Ag12225 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name UCHL5 interacting protein
Synonyms UIP1, UCHL5-interacting protein, UCHL5 interacting protein, HAUS7, HAUS augmin-like complex subunit 7
Calculated Molecular Weight 368 aa, 41 kDa
Observed Molecular Weight 46 kDa
GenBank Accession NumberBC008141
Gene Symbol UCHL5IP
Gene ID (NCBI) 55559
RRIDAB_2878447
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ99871
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for UCHL5IP antibody 17830-1-APDownload protocol
IHC protocol for UCHL5IP antibody 17830-1-APDownload protocol
IF protocol for UCHL5IP antibody 17830-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

J Cell Biol

Reconstitution and mechanistic dissection of the human microtubule branching machinery.

Authors - Yaqian Zhang
View Article