• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

Urocortin Polyclonal antibody

Urocortin Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 19731-1-AP

Print datasheet

Synonyms

UCN, UROC, UI

验证数据展示

  • WB analysis using 19731-1-AP

    WB analysis using 19731-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 19731-1-AP (Urocortin antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 19731-1-AP

    WB analysis of HeLa using 19731-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 19731-1-AP (Urocortin antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse kidney using 19731-1-AP

    IP experiment of mouse kidney using 19731-1-AP

    IP result of anti-Urocortin (IP:19731-1-AP, 4ug; Detection:19731-1-AP 1:1000) with mouse kidney tissue lysate 2160 ug.

经过测试的应用

Positive WB detected inmouse kidney tissue, HEK-293 cells, HeLa cells, human kidney tissue, human testis tissue
Positive IP detected inmouse kidney tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

19731-1-AP targets Urocortin in WB, IHC, IP, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IP, ELISA Application Description
Cited ApplicationsWB, IHC
Tested Reactivity human
Cited Reactivityhuman
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name urocortin
Synonyms UCN, UROC, UI
Calculated Molecular Weight 13 kDa
Observed Molecular Weight 22 kDa
GenBank Accession NumberNM_003353
Gene Symbol UCN
Gene ID (NCBI) 7349
RRIDAB_10640909
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP55089
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Urocortin is a member of the sauvagine/corticotropin-releasing factor/urotensin I family that includes CRF, urotensin I, sauvagine, urocortin II and urocortin III. Urocortin is a potent anorexigenic peptide that induces fed-like motor activity when administered centrally or peripherally in fasted animals. Urocortin is also a potent and long-lasting hypotensive agent and increases coronary blood flow. Urocortin acts in vitro to stimulate the secretion of adrenocorticotropic hormone (ACTH) and binds with high affinity to CRF receptor types 1, 2-alpha, and 2-beta. Urocortin is synthesized as precursor, which is subsequently processed to the mature biologically active peptide (a 40-amino acid Molecule ). The 22 kDa may be a urocortin 1 precursor protein (PMID: 28887029).

实验方案

Product Specific Protocols
WB protocol for Urocortin antibody 19731-1-APDownload protocol
IP protocol for Urocortin antibody 19731-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC,WB

J Cancer Res Clin Oncol

Urocortin-1 promotes colorectal cancer cell migration and proliferation and inhibits apoptosis via inhibition of the p53 signaling pathway

Authors - Xiaolan Guo
View Article