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UCP2 Polyclonal antibody
UCP2 Polyclonal Antibody for WB, IHC, FC (Intra), ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (1)
Applications
WB, IHC, FC (Intra), ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | mouse adipose tissue, mouse skeletal muscle tissue, mouse heart tissue, mouse spleen tissue, mouse liver tissue, rat liver tissue |
Positive IHC detected in | human cervical cancer tissue, human ovary tissue, human hepatocirrhosis tissue, human skeletal muscle tissue, human liver tissue, human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive FC (Intra) detected in | HeLa cells |
It is strongly recommended that fresh-made lysate be used for western blot assays.
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:1000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
11081-1-AP targets UCP2 in WB, IHC, IF, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | WB, IHC, FC (Intra), ELISA Application Description |
Cited Applications | WB, IHC, IF |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat, pig |
Immunogen | UCP2 fusion protein Ag1551 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | uncoupling protein 2 (mitochondrial, proton carrier) |
Synonyms | UCPH, UCP 2, SLC25A8, Dicarboxylate carrier SLC25A8, BMIQ4 |
Calculated Molecular Weight | 33 kDa |
Observed Molecular Weight | 28-30 kDa |
GenBank Accession Number | BC011737 |
Gene Symbol | UCP2 |
Gene ID (NCBI) | 7351 |
RRID | AB_2213793 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | P55851 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for UCP2 antibody 11081-1-AP | Download protocol |
IHC protocol for UCP2 antibody 11081-1-AP | Download protocol |
IP protocol for UCP2 antibody 11081-1-AP | Download protocol |
FC protocol for UCP2 antibody 11081-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Sci Transl Med Irisin protects mitochondria function during pulmonary ischemia/reperfusion injury. | ||
J Thromb Haemost The plasminogen receptor Plg-RKT regulates adipose function and metabolic homeostasis. | ||
EMBO Mol Med Disease-specific phenotypes in iPSC-derived neural stem cells with POLG mutations. | ||
Metabolism Far-infrared radiation prevents decline in β-cell mass and function in diabetic mice via the mitochondria-mediated Sirtuin1 pathway. | ||
Stem Cells UCP2 Regulates Embryonic Neurogenesis via ROS-Mediated Yap Alternation in the Developing Neocortex. | ||
Sci Rep Genipin inhibits NLRP3 and NLRC4 inflammasome activation via autophagy suppression. |