验证数据展示
经过测试的应用
| Positive WB detected in | mouse adipose tissue, mouse skeletal muscle tissue, mouse heart tissue, mouse spleen tissue, mouse liver tissue, rat liver tissue |
| Positive IHC detected in | human cervical cancer tissue, human ovary tissue, human hepatocirrhosis tissue, human skeletal muscle tissue, human liver tissue, human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive FC (Intra) detected in | HeLa cells |
It is strongly recommended that fresh-made lysate be used for western blot assays.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
11081-1-AP targets UCP2 in WB, IHC, IF, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, FC (Intra), ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat, pig |
| 免疫原 | UCP2 fusion protein Ag1551 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | uncoupling protein 2 (mitochondrial, proton carrier) |
| 别名 | UCPH, UCP 2, SLC25A8, Dicarboxylate carrier SLC25A8, BMIQ4 |
| 计算分子量 | 33 kDa |
| 观测分子量 | 28-30 kDa |
| GenBank蛋白编号 | BC011737 |
| 基因名称 | UCP2 |
| Gene ID (NCBI) | 7351 |
| RRID | AB_2213793 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P55851 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
UCP2 (uncoupling protein 2) is a member of the mitochondrial carrier protein super family located in the mitochondrial inner membrane. The UCPs differ greatly in tissue distribution: UCP1 is expressed only in brown adipose; UCP3 preferentially in skeletal muscle, brown adipose and heart; UCP4 and UCP5 mainly in the nervous system (18167556). In contract, UCP2 has a broad distribution and is implicated in a wide range of pathophysiological processes, including diabetes and cancer. This antibody detect a band around 28-33 kDa corresponding to UCP2 protein in mouse liver, skeletal muscle and pancreas. Some upper and lower bands also were observed due to the dimer formation and degradation of the UCP2 protein (20103532). This antibody was raised against the full-length UCP2 protein and may cross-react with UCP3. It is notable that UCP2 is known to have a very short half-life less than 1 hour (17240372). It is strongly recommended that fresh-made lysate be used for western blot assays.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for UCP2 antibody 11081-1-AP | Download protocol |
| IHC protocol for UCP2 antibody 11081-1-AP | Download protocol |
| IP protocol for UCP2 antibody 11081-1-AP | Download protocol |
| FC protocol for UCP2 antibody 11081-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Sci Transl Med Irisin protects mitochondria function during pulmonary ischemia/reperfusion injury. | ||
J Thromb Haemost The plasminogen receptor Plg-RKT regulates adipose function and metabolic homeostasis. | ||
EMBO Mol Med Disease-specific phenotypes in iPSC-derived neural stem cells with POLG mutations. | ||
Metabolism Far-infrared radiation prevents decline in β-cell mass and function in diabetic mice via the mitochondria-mediated Sirtuin1 pathway. | ||
Stem Cells UCP2 Regulates Embryonic Neurogenesis via ROS-Mediated Yap Alternation in the Developing Neocortex. | ||
Sci Rep Genipin inhibits NLRP3 and NLRC4 inflammasome activation via autophagy suppression. |
