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  • Featured Product
  • KD/KO Validated

UFD1L Polyclonal antibody

UFD1L Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 10615-1-AP

Print datasheet

Synonyms

UFD1, Ubiquitin recognition factor in ER-associated degradation protein 1, Ubiquitin fusion degradation protein 1, UB fusion protein 1

验证数据展示

  • WB analysis of K-562 using 10615-1-AP

    WB analysis of K-562 using 10615-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 10615-1-AP (UFD1L antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IP experiment of Raji using 10615-1-AP

    IP experiment of Raji using 10615-1-AP

    IP result of anti-UFD1L (IP:10615-1-AP, 4ug; Detection:10615-1-AP 1:1000) with Raji cells lysate 2000ug.

  • IF Staining of HeLa using 10615-1-AP

    IF Staining of HeLa using 10615-1-AP

    Immunofluorescent analysis of HeLa cells using 10615-1-AP (UFD1L antibody) at dilution of 1:25 and Alexa Fluor 594-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IF Staining of HepG2 using 10615-1-AP

    IF Staining of HepG2 using 10615-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using UFD1L antibody (10615-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • FC experiment of HEK-293 using 10615-1-AP

    FC experiment of HEK-293 using 10615-1-AP

    1X10^6 HEK-293 cells were intracellularly stained with 0.4 ug Anti-Human UFD1L (10615-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IHC staining of human stomach using 10615-1-AP

    IHC staining of human stomach using 10615-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 10615-1-AP (UFD1L antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with UFD1 antibody included

经过测试的应用

Positive WB detected inK-562 cells, mouse thymus tissue, Raji cells, HepG2 cells, human testis tissue
Positive IP detected inRaji cells
Positive IHC detected inhuman stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells, HeLa cells
Positive FC (Intra) detected inHEK-293 cells
Planning an IHC experiment? We recommend our IHCeasy UFD1 Ready-To-Use IHC Kit. UFD1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10615-1-AP targets UFD1L in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description
Cited ApplicationsWB, IHC, IF, IP
Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Immunogen UFD1L fusion protein Ag0954 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ubiquitin fusion degradation 1 like (yeast)
Synonyms UFD1, Ubiquitin recognition factor in ER-associated degradation protein 1, Ubiquitin fusion degradation protein 1, UB fusion protein 1
Calculated Molecular Weight 35 kDa
Observed Molecular Weight 35 kDa
GenBank Accession NumberBC005087
Gene Symbol UFD1L
Gene ID (NCBI) 7353
RRIDAB_2213944
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ92890
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for UFD1L antibody 10615-1-APDownload protocol
IHC protocol for UFD1L antibody 10615-1-APDownload protocol
IF protocol for UFD1L antibody 10615-1-APDownload protocol
IP protocol for UFD1L antibody 10615-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIP

Science

Ubiquitination of G3BP1 mediates stress granule disassembly in a context-specific manner.

Authors - Youngdae Gwon
View Article
humanWB

Nature

ESCRT-III controls nuclear envelope reformation.

Authors - Yolanda Olmos
  • KD Validated
View Article
humanWB

Dev Cell

The ESCRT machinery counteracts Nesprin-2G-mediated mechanical forces during nuclear envelope repair.

Authors - Samuel S Wallis
  • KD Validated
View Article
humanWB

Elife

The FAM104 proteins VCF1/2 promote the nuclear localization of p97/VCP

Authors - Maria Körner
View Article
mouseWB

Sci Signal

TRIM55 promotes noncanonical NF-κB signaling and B cell-mediated immune responses by coordinating p100 ubiquitination and processing

Authors - Liangbin Lin
View Article
WB

Plant J

The ubiquitin-26S proteasome system and autophagy relay proteome homeostasis regulation during silique development.

Authors - Peifeng Yu
View Article