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UFD1L Recombinant antibody

UFD1L Uni-rAbTM Recombinant Antibody for FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

230411A2

Cat no : 83669-1-RR

Print datasheet

Synonyms

UFD1, Ubiquitin recognition factor in ER-associated degradation protein 1, Ubiquitin fusion degradation protein 1, UB fusion protein 1, 230411A2

验证数据展示

  • FC experiment of HEK-293 using 83669-1-RR

    FC experiment of HEK-293 using 83669-1-RR

    1x10^6 HEK-293 cells were intracellularly stained with 0.25 ug Ufd1l Recombinant Antibody (83669-1-RR, Clone:230411A2) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • FC experiment of HeLa using 83669-1-RR

    FC experiment of HeLa using 83669-1-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug Ufd1l Recombinant Antibody (83669-1-RR, Clone:230411A2) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Affinity and Kinetic Characterization of 83669-1-RR

    Affinity and Kinetic Characterization of 83669-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83669-1-RR against Human UFD1L were performed. The affinity constant is below 1 pM.

经过测试的应用

Positive FC (Intra) detected inHEK-293 cells, HeLa cells
Planning an IHC experiment? We recommend our IHCeasy UFD1 Ready-To-Use IHC Kit. UFD1 primary antibody included.

推荐稀释比

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 83669-1-RR is UFD1L Fusion Protein expressed in E. coli.

Tested Applications FC (Intra), ELISA Application Description
Tested Reactivity human
Immunogen UFD1L fusion protein Ag0954 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name ubiquitin fusion degradation 1 like (yeast)
Synonyms UFD1, Ubiquitin recognition factor in ER-associated degradation protein 1, Ubiquitin fusion degradation protein 1, UB fusion protein 1, 230411A2
Calculated Molecular Weight 35 kDa
GenBank Accession NumberBC005087
Gene Symbol UFD1L
Gene ID (NCBI) 7353
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ92890
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
FC protocol for UFD1L antibody 83669-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols