验证数据展示
经过测试的应用
| Positive WB detected in | HT-29 cells, HEK-293 cells, HepG2 cells, mouse liver tissue, mouse lung tissue, L02 cells, U-251 cells |
| Positive IHC detected in | human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 4 publications below |
| IP | See 1 publications below |
产品信息
15883-1-AP targets UFM1 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Applications | WB, IHC, ELISA Application Description |
| Cited Applications | WB, IP |
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Immunogen | UFM1 fusion protein Ag8667 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | ubiquitin-fold modifier 1 |
| Synonyms | Ubiquitin-fold modifier 1, ubiquitin fold modifier 1, C13orf20, BM-002, BM 002 |
| Calculated Molecular Weight | 85 aa, 9 kDa |
| Observed Molecular Weight | 9-12 kDa |
| GenBank Accession Number | BC005193 |
| Gene Symbol | UFM1 |
| Gene ID (NCBI) | 51569 |
| RRID | AB_2878195 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P61960 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
UFM1 (Ubiquitin-fold modifier 1) is a ubiquitin-like protein covalently conjugated with intracellular proteins through UFMylation (modification by UFM1), a process similar to ubiquitylation (PMID: 38141606). UFM1 is conjugated to its target proteins by E1-like activating enzyme UBE1DC1 and E2-like conjugating enzyme UFC1 in a manner analogous to ubiquitylation (PMID: 28234446; 33066455). At the molecular level, UFMylation is an important mediator of the protein function. Dysregulation of the UFM1 system, e.g., the knockout of UFMylation components, disturbs proteome homeostasis and triggers endoplasmic reticulum stress. Such changes are linked to developmental disorders, tumorigenesis, tissue injury, inflammation, and several hereditary neurological syndromes (PMID: 36932998). Western blot images showed that bands larger than 100kd may be proteins modified by UFM1.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for UFM1 antibody 15883-1-AP | Download protocol |
| IHC protocol for UFM1 antibody 15883-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Mol Cell An Epstein-Barr virus protein interaction map reveals NLRP3 inflammasome evasion via MAVS UFMylation | ||
Mol Cell Proteomics Quantification Quality Control Emerges as A Crucial Factor to Enhance Single Cell Proteomics Data Analysis | ||
Autophagy VCP/p97 UFMylation stabilizes BECN1 and facilitates the initiation of autophagy | ||
