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UFSP2 Polyclonal antibody

UFSP2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 16999-1-AP

Print datasheet

Synonyms

C4orf20, UFM1 peptidase 2, Ufm1 specific protease 2, UFSP2

验证数据展示

  • WB analysis of mouse pancreas using 16999-1-AP

    WB analysis of mouse pancreas using 16999-1-AP

    mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 16999-1-AP (UFSP2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver using 16999-1-AP

    IHC staining of human liver using 16999-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 16999-1-AP (UFSP2 antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human liver using 16999-1-AP

    IHC staining of human liver using 16999-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 16999-1-AP (UFSP2 antibody) at dilution of 1:100 (under 40x lens).

  • IP experiment of HepG2 using 16999-1-AP

    IP experiment of HepG2 using 16999-1-AP

    IP result of anti-UFSP2 (IP:16999-1-AP, 3ug; Detection:16999-1-AP 1:1000) with HepG2 cells lysate 1700ug.

  • IF Staining of HeLa using 16999-1-AP

    IF Staining of HeLa using 16999-1-AP

    Immunofluorescent analysis of HeLa cells using 16999-1-AP (UFSP2 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inmouse pancreas tissue, NIH/3T3 cells
Positive IP detected inHepG2 cells
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16999-1-AP targets UFSP2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, IP, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen UFSP2 fusion protein Ag10692 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name UFM1-specific peptidase 2
Synonyms C4orf20, UFM1 peptidase 2, Ufm1 specific protease 2, UFSP2
Calculated Molecular Weight 469 aa, 53 kDa
Observed Molecular Weight 53 kDa
GenBank Accession NumberBC010493
Gene Symbol UFSP2
Gene ID (NCBI) 55325
RRIDAB_2214070
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NUQ7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Ubiquitin-fold modifier 1 (Ufm1)-specific protease 2 (UfSP2) is a cysteine protease that is responsible for the release of Ufm1 from Ufm1-conjugated cellular proteins, as well as for the generation of mature Ufm1 from its precursor (PMID: 21228277). The MW of this protein is 53 kDa, and this antibody specially recognises the 53 kDa protein.

实验方案

Product Specific Protocols
WB protocol for UFSP2 antibody 16999-1-APDownload protocol
IHC protocol for UFSP2 antibody 16999-1-APDownload protocol
IF protocol for UFSP2 antibody 16999-1-APDownload protocol
IP protocol for UFSP2 antibody 16999-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human,mouseWB

Genet Med

A pathogenic UFSP2 variant in an autosomal recessive form of pediatric neurodevelopmental anomalies and epilepsy.

Authors - Min Ni
View Article
humanWB

Sci Adv

The ufmylation cascade controls COPII recruitment, anterograde transport, and sorting of nascent GPCRs at ER

Authors - Xin Xu
  • KO Validated
View Article