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UGCG Polyclonal antibody
UGCG Polyclonal Antibody for WB, IHC, IF-P, IF-Fro, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF-P, IF-Fro, IP, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | A375 cells, C6 cells, mouse brain tissue, U-251 cells |
| Positive IP detected in | A375 cells |
| Positive IHC detected in | human lung cancer tissue, human breast cancer tissue, human lymphoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse kidney tissue, mouse brain tissue |
| Positive IF-Fro detected in | mouse kidney tissue |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Immunofluorescence (IF)-FRO | IF-FRO : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
12869-1-AP targets UGCG in WB, IHC, IF-P, IF-Fro, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, IHC, IF-P, IF-Fro, IP, ELISA Application Description |
| Cited Applications | WB, IHC, IF |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Immunogen | UGCG fusion protein Ag3530 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | UDP-glucose ceramide glucosyltransferase |
| Synonyms | Glycosylceramide synthase, Glucosylceramide synthase, GLCT-1, GCS, EC:2.4.1.80 |
| Calculated Molecular Weight | 394 aa, 45 kDa |
| Observed Molecular Weight | 50-55 kDa |
| GenBank Accession Number | BC038711 |
| Gene Symbol | UGCG |
| Gene ID (NCBI) | 7357 |
| RRID | AB_10915469 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q16739 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
UGCG, also named as GCS and GLCT-1, belongs to the glycosyltransferase 2 family. It catalyzes the first glycosylation step in glycosphingolipid biosynthesis, the transfer of glucose to ceramide. UGCG may also serve as a "flippase". It is up-regulated at the transcriptional level during keratinocyte differentiation. UGCG is a membrane protein. The calculated MW is 45 kDa. In SDS-PAGE, it is about 50-55 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for UGCG antibody 12869-1-AP | Download protocol |
| IHC protocol for UGCG antibody 12869-1-AP | Download protocol |
| IF protocol for UGCG antibody 12869-1-AP | Download protocol |
| IP protocol for UGCG antibody 12869-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun Glycolysis regulates KRAS plasma membrane localization and function through defined glycosphingolipids | ||
Cell Mol Biol Lett UGCG modulates heart hypertrophy through B4GalT5-mediated mitochondrial oxidative stress and the ERK signaling pathway | ||
Hum Mol Genet Amyotrophic lateral sclerosis and denervation alter sphingolipids and up-regulate glucosylceramide synthase. | ||
iScience Liver sphingomyelin synthase 1 deficiency causes steatosis, steatohepatitis, fibrosis, and tumorigenesis: An effect of glucosylceramide accumulation. |
