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UGCGL1 Monoclonal antibody

UGCGL1 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

3E8G10

Cat no : 68462-1-Ig

Print datasheet

Synonyms

FLJ23671, FLJ23796, GT, HUGT1, UGCGL1, UGGT, UGGT1, UGT1, UGTR

验证数据展示

  • WB analysis using 68462-1-Ig

    WB analysis using 68462-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68462-1-Ig (UGCGL1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inLNCaP cells, HeLa cells, HepG2 cells, Jurkat cells, human placenta tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68462-1-Ig targets UGCGL1 in WB, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity Human
Immunogen UGCGL1 fusion protein Ag5940 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name UDP-glucose ceramide glucosyltransferase-like 1
Synonyms FLJ23671, FLJ23796, GT, HUGT1, UGCGL1, UGGT, UGGT1, UGT1, UGTR
Calculated Molecular Weight 1531 aa, 175 kDa
Observed Molecular Weight170 kDa
GenBank Accession NumberBC041098
Gene Symbol UGCGL1
Gene ID (NCBI) 56886
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9NYU2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

UDP-glucose:glycoprotein glucosyltransferase 1 (UGCGL1) is a central quality control gatekeeper in the mammalian endoplasmic reticulum (ER) and serves as a folding sensor in the calnexin/calreticulin glycoprotein quality control cycle (PMID: 28425917). UGCGL1 acts as a checkpoint in the quality control of the MHC class I antigen presentation pathway (PMID: 21383159). Western blot analysis detected UGCGL1at an apparent molecular mass of 170 kDa.

实验方案

Product Specific Protocols
WB protocol for UGCGL1 antibody 68462-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols