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  • Featured Product
  • KD/KO Validated

UGDH Monoclonal antibody, PBS Only

UGDH Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat, pig

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1E2E1

Cat no : 67360-1-PBS

Print datasheet

Synonyms

GDH, UDP Glc dehydrogenase, UDP GlcDH, UDP glucose 6 dehydrogenase, UDP glucose dehydrogenase, UDPGDH, UGD, UGDH

验证数据展示

  • WB analysis using 67360-1-Ig (same clone as 67360-1-PBS)

    WB analysis using 67360-1-Ig (same clone as 67360-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67360-1-Ig (UGDH antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67360-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 67360-1-Ig (same clone as 67360-1-PBS)

    IHC staining of human liver cancer using 67360-1-Ig (same clone as 67360-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67360-1-Ig (UGDH antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67360-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 67360-1-Ig (same clone as 67360-1-PBS)

    IHC staining of human liver cancer using 67360-1-Ig (same clone as 67360-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67360-1-Ig (UGDH antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67360-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 67360-1-Ig (same clone as 67360-1-PBS)

    IF Staining of HepG2 using 67360-1-Ig (same clone as 67360-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using UGDH antibody (67360-1-Ig, Clone: 1E2E1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67360-1-PBS in a different storage buffer formulation.

产品信息

67360-1-PBS targets UGDH in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat, pig samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity Human, Mouse, Rat, pig
Immunogen UGDH fusion protein Ag29747 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name UDP-glucose dehydrogenase
Synonyms GDH, UDP Glc dehydrogenase, UDP GlcDH, UDP glucose 6 dehydrogenase, UDP glucose dehydrogenase, UDPGDH, UGD, UGDH
Calculated Molecular Weight 494 aa, 55 kDa
Observed Molecular Weight 55-60 kDa
GenBank Accession NumberBC022781
Gene Symbol UGDH
Gene ID (NCBI) 7358
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDO60701
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

UDP-glucose 6-dehydrogenase (UGDH) is a key enzyme in the uronic acid pathway, and converts UDP-glucose (UDP-Glc) to UDP-glucuronic acid (UDP-GlcA). UGDH is critical to the production of extracellular matrix components which are essential to the migration and connectivity of neurons early in human brain development. UDP-GlcA is an essential sugar nucleotide precursor and a key component in the synthesis and stepwise degradation of glycosaminoglycans (GAGs). The protein subunit migrates on SDS-PAGE at ~55 kDa. (PMID: 32175296, PMID: 21576248, PMID: 31243371).