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验证数据展示

  • WB analysis using 68381-1-Ig

    WB analysis using 68381-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68381-1-Ig (UGP2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis using 68381-1-Ig

    WB analysis using 68381-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68381-1-Ig (UGP2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 68381-1-Ig

    IHC staining of human liver cancer using 68381-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 68381-1-Ig (UGP2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

UGP2 Monoclonal antibody

UGP2 Monoclonal Antibody for WB, IHC, ELISA
Cat No. 68381-1-Ig

产品说明书

CloneNo. 1G11G6

宿主/亚型

Mouse / IgG2b

种属反应性

Human, mouse, Rat, Pig, Rabbit

应用

WB, IHC, ELISA

别名

pHC379, UDP glucose pyrophosphorylase, UDPG, UDPGP, UDPGP2, UGP1, UGP2, UGPase, UGPP2

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells, pig liver tissue, LNCaP cells, rabbit liver tissue, rat liver tissue, mouse liver tissue
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68381-1-Ig targets UGP2 in WB, IHC, ELISA applications and shows reactivity with Human, mouse, Rat, Pig, Rabbit samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 Human, mouse, Rat, Pig, Rabbit
免疫原 UGP2 fusion protein Ag33160 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 UDP-glucose pyrophosphorylase 2
别名 pHC379, UDP glucose pyrophosphorylase, UDPG, UDPGP, UDPGP2, UGP1, UGP2, UGPase, UGPP2
计算分子量 56 kDa
观测分子量 50-56 kDa
GenBank蛋白编号BC002954
基因名称 UGP2
Gene ID (NCBI) 7360
RRIDAB_3085099
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ16851
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

UDP-glucose pyrophosphorylase 2 (UGP2, synonyms: UDPG, UGPP2, UDPGP2, Phc379) is an important intermediary in mammalian carbohydrate interconversions. It transfers a glucose moiety from glucose-1-phosphate to MgUTP and forms UDP-glucose and MgPPi. In liver and muscle tissue, UDP-glucose is a direct precursor of glycogen; in lactating mammary gland it is converted to UDP-galactose which is then converted to lactose. So far two isoforms are encoded by two transcript variants have been found.

实验方案

Product Specific Protocols
WB protocol for UGP2 antibody 68381-1-IgDownload protocol
IHC protocol for UGP2 antibody 68381-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols