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UGT2B4 Polyclonal antibody

UGT2B4 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 15425-1-AP

Print datasheet

Synonyms

UDPGTh 1, UDPGT 2B4, UDP-glucuronosyltransferase 2B4, Hyodeoxycholic acid-specific UDPGT, HLUG25

验证数据展示

  • WB analysis of human liver using 15425-1-AP

    WB analysis of human liver using 15425-1-AP

    human liver tissue were subjected to SDS PAGE followed by western blot with 15425-1-AP (UGT2B4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver using 15425-1-AP

    IHC staining of human liver using 15425-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 15425-1-AP (UGT2B4 antibody) at dilution of 1:100 (under 10x lens).

    Ready-to-use IHC kits available with UGT2B4 antibody included
  • IHC staining of human liver using 15425-1-AP

    IHC staining of human liver using 15425-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 15425-1-AP (UGT2B4 antibody) at dilution of 1:100 (under 40x lens).

    Ready-to-use IHC kits available with UGT2B4 antibody included
  • IF Staining of HepG2 using 15425-1-AP

    IF Staining of HepG2 using 15425-1-AP

    Immunofluorescent analysis of HepG2 cells, using UGT2B4 antibody 15425-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

经过测试的应用

Positive WB detected inhuman liver tissue
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy UGT2B4 Ready-To-Use IHC Kit. UGT2B4 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

15425-1-AP targets UGT2B4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivityhuman
Immunogen UGT2B4 fusion protein Ag8058 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name UDP glucuronosyltransferase 2 family, polypeptide B4
Synonyms UDPGTh 1, UDPGT 2B4, UDP-glucuronosyltransferase 2B4, Hyodeoxycholic acid-specific UDPGT, HLUG25
Calculated Molecular Weight 60.5 kDa
Observed Molecular Weight 52-55 kDa
GenBank Accession NumberBC026264
Gene Symbol UGT2B4
Gene ID (NCBI) 7363
RRIDAB_2214247
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP06133
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for UGT2B4 antibody 15425-1-APDownload protocol
IHC protocol for UGT2B4 antibody 15425-1-APDownload protocol
IF protocol for UGT2B4 antibody 15425-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Drug Metab Dispos

Upregulation of UGT2B4 Expression by 3'-Phosphoadenosine 5'-Phosphosulfate Synthase Knockdown: Implications for Coordinated Control of Bile Acid Conjugation.

Authors - Kathleen G Barrett
View Article