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UGT3A2 Polyclonal antibody

UGT3A2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 21366-1-AP

Print datasheet

Synonyms

UDPGT 3A2, UGT3A2, UNQ842/PRO1780

验证数据展示

  • WB analysis using 21366-1-AP

    WB analysis using 21366-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21366-1-AP (UGT3A2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse liver using 21366-1-AP

    IHC staining of mouse liver using 21366-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 21366-1-AP (UGT3A2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inMOLT-4 cells, human testis tissue, THP-1 cells
Positive IHC detected inmouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IHCSee 1 publications below

产品信息

21366-1-AP targets UGT3A2 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsIHC
Tested Reactivity human, mouse
Immunogen UGT3A2 fusion protein Ag15835 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name UDP glycosyltransferase 3 family, polypeptide A2
Synonyms UDPGT 3A2, UGT3A2, UNQ842/PRO1780
Calculated Molecular Weight 523 aa, 60 kDa
Observed Molecular Weight 50-55 kDa
GenBank Accession NumberBC103925
Gene Symbol UGT3A2
Gene ID (NCBI) 167127
RRIDAB_10888629
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ3SY77
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for UGT3A2 antibody 21366-1-APDownload protocol
IHC protocol for UGT3A2 antibody 21366-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
IHC

Front Mol Biosci

Identification of small extracellular vesicle protein biomarkers for pediatric Ewing Sarcoma

Authors - Soumya M Turaga
View Article