验证数据展示
经过测试的应用
| Positive WB detected in | mouse thymus tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | human stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
11624-1-AP targets UHMK1 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, rat |
| 免疫原 | UHMK1 fusion protein Ag2204 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | U2AF homology motif (UHM) kinase 1 |
| 别名 | Serine/threonine-protein kinase Kist, P-CIP2, PAM COOH-terminal interactor protein 2, KIST, KIS |
| 计算分子量 | 419 aa, 47 kDa |
| 观测分子量 | 47 kDa |
| GenBank蛋白编号 | BC026046 |
| 基因名称 | UHMK1 |
| Gene ID (NCBI) | 127933 |
| RRID | AB_2214267 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q8TAS1 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
UHMK1, also named as KIS, or KIST, is a 419 amino acid protein, which contains one RRM (RNA recognition motif) domain, one protein kinase domain, and belongs to the protein kinase superfamily. UHMK1 is widely expressed, with highest levels in skeletal muscle, kidney, placenta and peripheral blood leukocytes. UHMK1 localizes in the nucleus and upon serum stimulation, phosphorylating CDKN1B/p27Kip1, thus controlling CDKN1B subcellular location and cell cycle progression in G1 phase. UHMK1 may be involved in trafficking and/or processing of RNA and may have a role in the developing nervous system and the adult brain.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for UHMK1 antibody 11624-1-AP | Download protocol |
| IHC protocol for UHMK1 antibody 11624-1-AP | Download protocol |
| IP protocol for UHMK1 antibody 11624-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Brain Res Cord blood administration induces oligodendrocyte survival through alterations in gene expression. | ||
Cell Death Dis UHMK1 aids colorectal cancer cell proliferation and chemoresistance through augmenting IL-6/STAT3 signaling.
| ||
Thorac Cancer UHMK1 promotes lung adenocarcinoma oncogenesis by regulating the PI3K/AKT/mTOR signaling pathway
| ||
J Cancer Res Clin Oncol KIS, a target of SOX4, regulates the ID1-mediated enhancement of β-catenin to facilitate lung adenocarcinoma cell proliferation and metastasis
| ||
Oral Surg Oral Med Oral Pathol Oral Radiol Expression and clinical significance of U2AF homology motif kinase 1 in oral squamous cell carcinoma |