UHRF2 Polyclonal antibody
UHRF2 Polyclonal Antibody for WB, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
WB, IF/ICC, IP, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | HL-60 cells, HeLa cells, Jurkat cells, PC-3 cells |
Positive IP detected in | Jurkat cells |
Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
Application | Dilution |
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Western Blot (WB) | WB : 1:500-1:2000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 1 publications below |
产品信息
25710-1-AP targets UHRF2 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human samples.
Tested Applications | WB, IF/ICC, IP, ELISA Application Description |
Cited Applications | WB |
Tested Reactivity | human |
Cited Reactivity | human |
Immunogen | UHRF2 fusion protein Ag22519 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | ubiquitin-like with PHD and ring finger domains 2 |
Synonyms | NIRF, Nuclear protein 97, RING finger protein 107, RNF107, UHRF2, URF2 |
Calculated Molecular Weight | 90 kDa |
Observed Molecular Weight | 90 kDa |
GenBank Accession Number | BC028397 |
Gene Symbol | UHRF2 |
Gene ID (NCBI) | 115426 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q96PU4 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Ubiquitin-Like with PHD and ring finger domains 2 (UHRF2), a member that belongs to the family of UHRF, contains five recognizable functional domains, namely the ubiquitin-like domain (UBL) domain, tandem-Tudor domain (TTD), plant homeodomain (PHD), SET and RING associated (SRA) domain, and really interesting new gene (RING) finger domain. Due to the complex structure, UHRF2 possesses multiple functions in diverse cellular processes. As a ubiquitin E3 ligase, UHRF2 could ubiquitinate PCNP, a nuclear protein that contains two remarkable PEST sequences which are rich in proline (P), glutamic acid (E), serine (S), and threonine (T). It has been also reported that UHRF2 could serve as a vital cell cycle regulator by interacting with multiple cyclins, CDKs, p53, pRB and PCNA. UHRF2 has been revealed to possess epigenetic regulation function and is capable of maintaining 5mC levels in certain genomic loci in brain and stabilizes TIP60 to regulate H3K9ac and H3K14ac through RING finger domain. Moreover, UHRF2 could promote DNA damage repair by reducing the level of p21 mediated by RING finger domain. Recently, emerging evidence indicated that UHRF2 was involved in the tumorigenesis and progression of several human cancers, such as esophageal squamous cell carcinoma, lung cancer and colorectal cancer. UHRF2 has 2 isoforms with the molecular mass of 56 and 90 kDa. (PMID: 34400880)
实验方案
Product Specific Protocols | |
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WB protocol for UHRF2 antibody 25710-1-AP | Download protocol |
IF protocol for UHRF2 antibody 25710-1-AP | Download protocol |
IP protocol for UHRF2 antibody 25710-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |