UIMC1 Polyclonal antibody

UIMC1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 13642-1-AP

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Synonyms

Ubiquitin interaction motif-containing protein 1, RXRIP110, Retinoid X receptor-interacting protein 110, Receptor-associated protein 80, RAP80



经过测试的应用

Positive WB detected inmouse colon tissue, COLO 320 cells, HeLa cells, Raji cells
Positive IP detected inCOLO 320 cells
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHela cells, MCF-7 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13642-1-AP targets UIMC1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF/ICC, IP, ELISA Application Description
Tested Reactivity human, mouse
Immunogen UIMC1 fusion protein Ag4582 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ubiquitin interaction motif containing 1
Synonyms Ubiquitin interaction motif-containing protein 1, RXRIP110, Retinoid X receptor-interacting protein 110, Receptor-associated protein 80, RAP80
Calculated Molecular Weight 719 aa, 80 kDa
Observed Molecular Weight 97 kDa, 80 kDa, 71-75 kDa, 61 kDa
GenBank Accession NumberBC032561
Gene Symbol UIMC1
Gene ID (NCBI) 51720
RRIDAB_10641052
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96RL1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for UIMC1 antibody 13642-1-APDownload protocol
IHC protocol for UIMC1 antibody 13642-1-APDownload protocol
IF protocol for UIMC1 antibody 13642-1-APDownload protocol
IP protocol for UIMC1 antibody 13642-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols