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UIMC1 Polyclonal antibody

UIMC1 Polyclonal Antibody for IF/ICC, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC, IHC, IP, WB,ELISA

Conjugate

Unconjugated

Cat No : 13642-1-AP

Print datasheet

Synonyms

BRCA1 A complex subunit RAP80, RAP80, Receptor associated protein 80, RXRIP110, UIMC1, X2HRIP110

验证数据展示

  • WB analysis of mouse colon using 13642-1-AP

    WB analysis of mouse colon using 13642-1-AP

    mouse colon tissue were subjected to SDS PAGE followed by western blot with 13642-1-AP (UIMC1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 13642-1-AP

    IF Staining of HeLa using 13642-1-AP

    Immunofluorescent analysis of Hela cells, using UIMC1 antibody 13642-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

  • IP experiment of COLO 320 using 13642-1-AP

    IP experiment of COLO 320 using 13642-1-AP

    IP result of anti-UIMC1 (IP:13642-1-AP, 4ug; Detection:13642-1-AP 1:500) with COLO 320 cells lysate 2400ug.

  • IHC staining of human testis using 13642-1-AP

    IHC staining of human testis using 13642-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 13642-1-AP (UIMC1 Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human testis using 13642-1-AP

    IHC staining of human testis using 13642-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 13642-1-AP (UIMC1 Antibody) at dilution of 1:50 (under 40x lens).

经过测试的应用

Positive WB detected inmouse colon tissue, COLO 320 cells, HeLa cells, Raji cells
Positive IP detected inCOLO 320 cells
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHela cells, MCF-7 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13642-1-AP targets UIMC1 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications IF/ICC, IHC, IP, WB,ELISA Application Description
Tested Reactivity human, mouse
Immunogen UIMC1 fusion protein Ag4582 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ubiquitin interaction motif containing 1
Synonyms BRCA1 A complex subunit RAP80, RAP80, Receptor associated protein 80, RXRIP110, UIMC1, X2HRIP110
Calculated Molecular Weight 719 aa, 80 kDa
Observed Molecular Weight 97 kDa, 80 kDa, 71-75 kDa, 61 kDa
GenBank Accession NumberBC032561
Gene Symbol UIMC1
Gene ID (NCBI) 51720
RRIDAB_10641052
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96RL1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for UIMC1 antibody 13642-1-APDownload protocol
IHC protocol for UIMC1 antibody 13642-1-APDownload protocol
IF protocol for UIMC1 antibody 13642-1-APDownload protocol
IP protocol for UIMC1 antibody 13642-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols