• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 83709-1-RR

    WB analysis using 83709-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83709-1-RR (ULBP2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 83709-1-RR

    IHC staining of human colon cancer using 83709-1-RR

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 83709-1-RR (ULBP2 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ULBP2 antibody included
  • IHC staining of human colon cancer using 83709-1-RR

    IHC staining of human colon cancer using 83709-1-RR

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 83709-1-RR (ULBP2 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ULBP2 antibody included
  • Affinity and Kinetic Characterization of 83709-1-RR

    Affinity and Kinetic Characterization of 83709-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83709-1-RR against Human ULBP2 were performed. The affinity constant is below 1 pM.

ULBP2 Recombinant antibody

ULBP2 Uni-rAbTM Recombinant Antibody for WB, IHC, ELISA
Cat No. 83709-1-RR

产品说明书

CloneNo. 240413A6

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

NKG2D ligand 2, N2DL-2, N2DL2, ALCAN-alpha, ALCAN alpha

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inJurkat cells, Saos-2 cells, THP-1 cells, COLO 320 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy ULBP2 Ready-To-Use IHC Kit. ULBP2 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83709-1-RR targets ULBP2 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原 Fusion Protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 UL16 binding protein 2
别名 NKG2D ligand 2, N2DL-2, N2DL2, ALCAN-alpha, ALCAN alpha
计算分子量27 kDa
观测分子量30 kDa
GenBank蛋白编号NM_025217.4
基因名称 ULBP2
Gene ID (NCBI) 80328
RRIDAB_3671312
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ9BZM5
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NKG2D is an activating cell surface receptor that is predominantly expressed on cytotoxic immune cells. NKG2D recognizes a wide range of ligands. In humans, the NKG2D ligand (NKG2DL) includes MICA, MICB, and six members of the ULBP family (ULBP1-6) (PMID: 29568297; 30813924). ULBPs are MHC class I-related molecules (PMID: 11239445). ULBP2 contains two Ig-like domains, the alpha-1 and alpha-2 domains characteristic of the MHC class I family, but lacks the alpha-3 domain. It can be expressed as either a transmembrane or GPI-linked protein, or released from the cell surface (PMID: 21224393; 24614922). ULBP2 binds and activates the NKG2D, mediating the recruitment and activation of NK cells.

实验方案

Product Specific Protocols
WB protocol for ULBP2 antibody 83709-1-RRDownload protocol
IHC protocol for ULBP2 antibody 83709-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols