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验证数据展示

  • WB analysis using 27352-1-AP

    WB analysis using 27352-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27352-1-AP (ULK1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis using 27352-1-AP

    WB analysis using 27352-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27352-1-AP (ULK1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse skeletal muscle using 27352-1-AP

    IHC staining of mouse skeletal muscle using 27352-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 27352-1-AP (ULK1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ULK1 antibody included
  • IHC staining of mouse skeletal muscle using 27352-1-AP

    IHC staining of mouse skeletal muscle using 27352-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 27352-1-AP (ULK1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ULK1 antibody included
  • IF Staining of HeLa using 27352-1-AP

    IF Staining of HeLa using 27352-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using ULK1 antibody (27352-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

ULK1 Polyclonal antibody

ULK1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 27352-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

Serine/threonine-protein kinase ULK1, KIAA0722, hATG1, EC:2.7.11.1, Autophagy-related protein 1 homolog

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, C2C12 cells, HepG2 cells, HSC-T6 cellls
Positive IHC detected inmouse skeletal muscle tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy ULK1 Ready-To-Use IHC Kit. ULK1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27352-1-AP targets ULK1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB, IHC
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原 ULK1 fusion protein Ag22397 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 unc-51-like kinase 1 (C. elegans)
别名 Serine/threonine-protein kinase ULK1, KIAA0722, hATG1, EC:2.7.11.1, Autophagy-related protein 1 homolog
观测分子量 140-150 kDa
GenBank蛋白编号NM_003565
基因名称 ULK1
Gene ID (NCBI) 8408
RRIDAB_3085951
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO75385
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ULK1, also named as KIAA0722, belongs to the protein kinase superfamily, Ser/Thr protein kinase family and APG1/unc-51/ULK1 subfamily. It is involved in autophagy. ULK1 is required for autophagosome formation. It forms a stable complex with Atg13 and focal adhesion kinase (FAK) family interacting protein of 200 kDa (FIP 200)(PMID:21460634). ULK1 phosphorylates ATG13/KIAA0652. It is involved in axon growth. ULK1 plays an essential role in neurite extension of cerebellar granule cells. ULK1 represents a potential novel prognostic biomarker for HCC patients and may play an important role during the progression of HCC(PMID:23573318). ULK1 is a 113 kDa protein that it also can be detected a band of 150 kDa through the western blot (PMID: 32904616, PMID: 32899862, PMID: 37270528).

实验方案

Product Specific Protocols
WB protocol for ULK1 antibody 27352-1-APDownload protocol
IHC protocol for ULK1 antibody 27352-1-APDownload protocol
IF protocol for ULK1 antibody 27352-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IHC

Mol Carcinog

Zinc Finger Protein 263 Augments Autophagy and Promotes Intrahepatic Cholangiocarcinoma Proliferation

Authors - Zaihua Yan
View Article