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验证数据展示

  • WB analysis of human urine using 29975-1-AP

    WB analysis of human urine using 29975-1-AP

    human urine sample were subjected to SDS PAGE followed by western blot with 29975-1-AP (Uromodulin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 29975-1-AP

    IHC staining of human kidney using 29975-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 29975-1-AP (Uromodulin antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human kidney using 29975-1-AP

    IHC staining of human kidney using 29975-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 29975-1-AP (Uromodulin antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse kidney using 29975-1-AP

    IF Staining of mouse kidney using 29975-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse kidney tissue using Uromodulin antibody (29975-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • IF Staining of mouse kidney using 29975-1-AP

    IF Staining of mouse kidney using 29975-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse kidney tissue using Uromodulin antibody (29975-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

Uromodulin Polyclonal antibody

Uromodulin Polyclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 29975-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human, Mouse

应用

WB, IHC, IF-P, ELISA

别名

ADMCKD2, FJHN, HNFJ, MCKD2, THGP, THP, UMOD, uromodulin

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inhuman urine sample
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse kidney tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:5000-1:20000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29975-1-AP targets Uromodulin in WB, IHC, IF-P, ELISA applications and shows reactivity with Human, Mouse samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
经测试反应性 Human, Mouse
免疫原 Uromodulin fusion protein Ag32343 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 uromodulin
别名 ADMCKD2, FJHN, HNFJ, MCKD2, THGP, THP, UMOD, uromodulin
计算分子量 640 aa, 70 kDa
观测分子量80-90 kDa
GenBank蛋白编号BC035975
基因名称 Uromodulin
Gene ID (NCBI) 7369
RRIDAB_2935492
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP07911
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Uromodulin, also known as THP (Tamm-Horsfall glycoprotein), is the most abundant protein excreted in the urine under physiological conditions. Uromodulin is synthesized by the kidney and localizes in cells lining the ascending limb of Henle and distal convoluted tubule. Uromodulin has been linked to water/electrolyte balance and to kidney innate immunity. Also, studies in knockout mice demonstrated that it has a protective role against urinary tract infections and renal stone formation. Mutations in the gene encoding uromodulin lead to rare autosomal dominant diseases, collectively referred to as uromodulin-associated kidney diseases. (PMID: 21654721)

实验方案

Product Specific Protocols
WB protocol for Uromodulin antibody 29975-1-APDownload protocol
IHC protocol for Uromodulin antibody 29975-1-APDownload protocol
IF protocol for Uromodulin antibody 29975-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols