验证数据展示
经过测试的应用
| Positive WB detected in | mouse brain tissue, human brain tissue, rat brain tissue |
| Positive IP detected in | mouse brain tissue |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
55053-1-AP targets UNC13A in WB, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, IP, ELISA Application Description |
| Cited Applications | WB, IF, CoIP |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Immunogen | Peptide 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | unc-13 homolog A (C. elegans) |
| Synonyms | UNC13A/Munc13-1, Munc13-1, unc 13 homolog A (C. elegans), Protein unc-13 homolog A, Protein unc 13 homolog A |
| Calculated Molecular Weight | 193 kDa |
| Observed Molecular Weight | 193-210 kDa |
| GenBank Accession Number | NM_001080421 |
| Gene Symbol | UNC13A |
| Gene ID (NCBI) | 23025 |
| RRID | AB_10804173 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9UPW8 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
UNC13A, also named KIAA1032, belongs to the unc-13 family. It plays a role in vesicle maturation during exocytosis as a target of the diacylglycerol second messenger pathway. UNC13A is involved in neurotransmitter release by acting in synaptic vesicle priming prior to vesicle fusion and participates in the activity-dependent refilling of readily releasable vesicle pool (RRP). It is essential for synaptic vesicle maturation in most excitatory/glutamatergic but not inhibitory/GABA-mediated synapses. UNC13A is also involved in secretory granule priming in INS secretion. The antibody is specific to UNC13A.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for UNC13A antibody 55053-1-AP | Download protocol |
| IP protocol for UNC13A antibody 55053-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Elife Exophilin-8 assembles secretory granules for exocytosis in the actin cortex via interaction with RIM-BP2 and myosin-VIIa. | ||
Cell Rep Structural and Functional Analysis of the CAPS SNARE-Binding Domain Required for SNARE Complex Formation and Exocytosis. | ||
Sci Adv Phosphorylation of Doc2 by EphB2 modulates Munc13-mediated SNARE complex assembly and neurotransmitter release |
