UNC13A Polyclonal antibody

UNC13A Polyclonal Antibody for WB, IP, ELISA
Cat No. 55053-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IP, ELISA and More (2)

UNC13A/Munc13-1, Munc13-1, unc 13 homolog A (C. elegans), Protein unc-13 homolog A, Protein unc 13 homolog A

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inmouse brain tissue, human brain tissue, rat brain tissue
Positive IP detected inmouse brain tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

55053-1-AP targets UNC13A in WB, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IP, ELISA Application Description
文献引用应用WB, IF, CoIP
经测试反应性 human, mouse, rat
文献引用反应性human, mouse
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 unc-13 homolog A (C. elegans)
别名 UNC13A/Munc13-1, Munc13-1, unc 13 homolog A (C. elegans), Protein unc-13 homolog A, Protein unc 13 homolog A
计算分子量 193 kDa
观测分子量 193-210 kDa
GenBank蛋白编号NM_001080421
基因名称 UNC13A
Gene ID (NCBI) 23025
RRIDAB_10804173
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UPW8
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

UNC13A, also named KIAA1032, belongs to the unc-13 family. It plays a role in vesicle maturation during exocytosis as a target of the diacylglycerol second messenger pathway. UNC13A is involved in neurotransmitter release by acting in synaptic vesicle priming prior to vesicle fusion and participates in the activity-dependent refilling of readily releasable vesicle pool (RRP). It is essential for synaptic vesicle maturation in most excitatory/glutamatergic but not inhibitory/GABA-mediated synapses. UNC13A is also involved in secretory granule priming in INS secretion. The antibody is specific to UNC13A.

实验方案

Product Specific Protocols
WB protocol for UNC13A antibody 55053-1-APDownload protocol
IP protocol for UNC13A antibody 55053-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Nature

TDP-43 represses cryptic exon inclusion in the FTD-ALS gene UNC13A.

Authors - X Rosa Ma
mouseWB

Elife

Exophilin-8 assembles secretory granules for exocytosis in the actin cortex via interaction with RIM-BP2 and myosin-VIIa.

Authors - Fushun Fan
CoIP

Cell Rep

Structural and Functional Analysis of the CAPS SNARE-Binding Domain Required for SNARE Complex Formation and Exocytosis.

Authors - Hao Zhou
human,mouseWB,IF

Sci Adv

Phosphorylation of Doc2 by EphB2 modulates Munc13-mediated SNARE complex assembly and neurotransmitter release

Authors - Hong Zhang
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