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UNC13D/Munc13-4 Polyclonal antibody

UNC13D/Munc13-4 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IHC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 16905-1-AP

Print datasheet

Synonyms

FHL3, HLH3, HPLH3, Munc13 4, Munc13-4, Protein unc 13 homolog D, unc 13 homolog D (C. elegans), UNC13D, UNC13D/Munc13-4

验证数据展示

  • WB analysis using 16905-1-AP

    WB analysis using 16905-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16905-1-AP (UNC13D/Munc13-4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis of K-562 using 16905-1-AP

    WB analysis of K-562 using 16905-1-AP

    WB result of UNC13D/Munc13-4 antibody (16905-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-UNC13D/Munc13-4 transfected K-562 cells.

  • IHC staining of human thymus using 16905-1-AP

    IHC staining of human thymus using 16905-1-AP

    Immunohistochemical analysis of paraffin-embedded human thymus tissue slide using 16905-1-AP (UNC13D/Munc13-4 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with UNC13D antibody included
  • IHC staining of human thymus using 16905-1-AP

    IHC staining of human thymus using 16905-1-AP

    Immunohistochemical analysis of paraffin-embedded human thymus tissue slide using 16905-1-AP (UNC13D/Munc13-4 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with UNC13D antibody included
  • IP experiment of Jurkat using 16905-1-AP

    IP experiment of Jurkat using 16905-1-AP

    IP result of anti-UNC13D/Munc13-4 (IP:16905-1-AP, 5ug; Detection:16905-1-AP 1:300) with Jurkat cells lysate 2400ug.

经过测试的应用

Positive WB detected inJurkat cells, K-562 cells, human placenta tissue
Positive IP detected inJurkat cells
Positive IHC detected inhuman thymus tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy UNC13D Ready-To-Use IHC Kit. UNC13D primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16905-1-AP targets UNC13D/Munc13-4 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IP, IHC, ELISA Application Description
Cited ApplicationsWB, IF
Tested Reactivity human
Cited Reactivityhuman
Immunogen UNC13D/Munc13-4 fusion protein Ag10519 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name unc-13 homolog D (C. elegans)
Synonyms FHL3, HLH3, HPLH3, Munc13 4, Munc13-4, Protein unc 13 homolog D, unc 13 homolog D (C. elegans), UNC13D, UNC13D/Munc13-4
Calculated Molecular Weight 1090 aa, 103 kDa
Observed Molecular Weight 120 kDa
GenBank Accession NumberBC067084
Gene Symbol UNC13D
Gene ID (NCBI) 201294
RRIDAB_2212713
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ70J99
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for UNC13D/Munc13-4 antibody 16905-1-APDownload protocol
IHC protocol for UNC13D/Munc13-4 antibody 16905-1-APDownload protocol
IP protocol for UNC13D/Munc13-4 antibody 16905-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Mol Cell

Integrative omics indicate FMRP sequesters mRNA from translation and deadenylation in human neuronal cells

Authors - Tatsuaki Kurosaki
View Article
humanWB

J Exp Med

Transcriptional regulation of Munc13-4 expression in cytotoxic lymphocytes is disrupted by an intronic mutation associated with a primary immunodeficiency.

Authors - Frank Cichocki
View Article
humanWB

Blood

Familial hemophagocytic lymphohistiocytosis type 3 (FHL3) caused by deep intronic mutation and inversion in UNC13D.

Authors - Meeths Marie M
View Article
humanWB

Front Immunol

Alternative UNC13D Promoter Encodes a Functional Munc13-4 Isoform Predominantly Expressed in Lymphocytes and Platelets.

Authors - Donatella Galgano
View Article
humanWB,IF

Mol Biol Cell

A novel Munc13-4/S100A10/Annexin A2 complex promotes Weibel-Palade body exocytosis in endothelial cells.

Authors - Tarek Chehab
View Article