UNC13D/Munc13-4 Polyclonal antibody

UNC13D/Munc13-4 Polyclonal Antibody for WB, IP, IHC, ELISA
Cat No. 16905-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IP, IHC, ELISA and More (1)

FHL3, HLH3, HPLH3, Munc13 4, Munc13-4, Protein unc 13 homolog D, unc 13 homolog D (C. elegans), UNC13D, UNC13D/Munc13-4

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inJurkat cells, K-562 cells, human placenta tissue
Positive IP detected inJurkat cells
Positive IHC detected inhuman thymus tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy UNC13D Ready-To-Use IHC Kit. UNC13D primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16905-1-AP targets UNC13D/Munc13-4 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IP, IHC, ELISA Application Description
文献引用应用WB, IF
经测试反应性 human
文献引用反应性human
免疫原 UNC13D/Munc13-4 fusion protein Ag10519 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 unc-13 homolog D (C. elegans)
别名 FHL3, HLH3, HPLH3, Munc13 4, Munc13-4, Protein unc 13 homolog D, unc 13 homolog D (C. elegans), UNC13D, UNC13D/Munc13-4
计算分子量 1090 aa, 103 kDa
观测分子量 120 kDa
GenBank蛋白编号BC067084
基因名称 UNC13D
Gene ID (NCBI) 201294
RRIDAB_2212713
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ70J99
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for UNC13D/Munc13-4 antibody 16905-1-APDownload protocol
IHC protocol for UNC13D/Munc13-4 antibody 16905-1-APDownload protocol
IP protocol for UNC13D/Munc13-4 antibody 16905-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Mol Cell

Integrative omics indicate FMRP sequesters mRNA from translation and deadenylation in human neuronal cells

Authors - Tatsuaki Kurosaki
humanWB

J Exp Med

Transcriptional regulation of Munc13-4 expression in cytotoxic lymphocytes is disrupted by an intronic mutation associated with a primary immunodeficiency.

Authors - Frank Cichocki
humanWB

Blood

Familial hemophagocytic lymphohistiocytosis type 3 (FHL3) caused by deep intronic mutation and inversion in UNC13D.

Authors - Meeths Marie M
humanWB

Front Immunol

Alternative UNC13D Promoter Encodes a Functional Munc13-4 Isoform Predominantly Expressed in Lymphocytes and Platelets.

Authors - Donatella Galgano
humanWB,IF

Mol Biol Cell

A novel Munc13-4/S100A10/Annexin A2 complex promotes Weibel-Palade body exocytosis in endothelial cells.

Authors - Tarek Chehab
humanWB,IF

J Transl Med

Recycling machinery of integrin coupled with focal adhesion turnover via RAB11-UNC13D-FAK axis for migration of pancreatic cancer cells

Authors - Van-Thanh Duong
Loading...