验证数据展示
经过测试的应用
| Positive WB detected in | K-562 cells, mouse lung tissue, mouse ovary tissue, HeLa cells |
| Positive IP detected in | K-562 cells |
| Positive IHC detected in | human ovary cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
19564-1-AP targets UNC45A in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | unc-45 homolog A (C. elegans) |
| 别名 | Protein unc 45 homolog A, IRO039700, GCUNC-45, GCUNC45, GCUNC 45 |
| 计算分子量 | 103 kDa |
| 观测分子量 | 100 kDa |
| GenBank蛋白编号 | NM_018671 |
| 基因名称 | UNC45A |
| Gene ID (NCBI) | 55898 |
| RRID | AB_10644335 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9H3U1 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
UNC45A, also named as SMAP1, acts as co-chaperone for HSP90. UNC45A prevents the stimulation of HSP90AB1 ATPase activity by AHSA1. It is a positive factor in promoting PGR function in the cell. It is necessary for proper folding of myosin and necessary for normal cell proliferation, normal myotube formation and myosin accumulation during muscle cell development. UNC45A plays a role in erythropoiesis in stroma cells in the spleen. The antibody recognizes the C-term of UNC45A.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for UNC45A antibody 19564-1-AP | Download protocol |
| IHC protocol for UNC45A antibody 19564-1-AP | Download protocol |
| IP protocol for UNC45A antibody 19564-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
FASEB J A myosin chaperone, UNC-45A, is a novel regulator of intestinal epithelial barrier integrity and repair.
| ||
J Cell Sci UNC-45A breaks the microtubule lattice independently of its effects on non-muscle myosin II. | ||
Cells UNC-45A Is Highly Expressed in the Proliferative Cells of the Mouse Genital Tract and in the Microtubule-Rich Areas of the Mouse Nervous System. |
