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UNC45B Polyclonal antibody

UNC45B Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 21640-1-AP

Print datasheet

Synonyms

CMYA4, Protein unc 45 homolog B, SMUNC45, unc 45 homolog B (C. elegans), Unc 45B, UNC45, unc-45 myosin chaperone B, UNC45B

验证数据展示

  • WB analysis using 21640-1-AP

    WB analysis using 21640-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21640-1-AP (UNC45B antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse heart using 21640-1-AP

    IHC staining of mouse heart using 21640-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 21640-1-AP (UNC45B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inmouse skeletal muscle tissue, rat skeletal muscle tissue
Positive IHC detected inmouse heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

21640-1-AP targets UNC45B in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Immunogen UNC45B fusion protein Ag16294 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name unc-45 homolog B (C. elegans)
Synonyms CMYA4, Protein unc 45 homolog B, SMUNC45, unc 45 homolog B (C. elegans), Unc 45B, UNC45, unc-45 myosin chaperone B, UNC45B
Calculated Molecular Weight 931 aa, 104 kDa
Observed Molecular Weight 95 kDa
GenBank Accession NumberBC101063
Gene Symbol UNC45B
Gene ID (NCBI) 146862
RRIDAB_10859787
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8IWX7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for UNC45B antibody 21640-1-APDownload protocol
IHC protocol for UNC45B antibody 21640-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Mol Biol Cell

The myosin chaperone UNC-45 has an important role in maintaining the structure and function of muscle sarcomeres during adult aging

Authors - Courtney J Matheny
View Article