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验证数据展示

  • WB analysis using 28359-1-AP

    WB analysis using 28359-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28359-1-AP (UNC93B1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary cancer using 28359-1-AP

    IHC staining of human ovary cancer using 28359-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 28359-1-AP (UNC93B1 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

UNC93B1 Polyclonal antibody

UNC93B1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 28359-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat, pig

应用

WB, IHC, ELISA

别名

UNC93, Unc 93B1, unc 93 homolog B1 (C. elegans), Protein unc-93 homolog B1, Protein unc 93 homolog B1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse spleen tissue, rat kidney tissue, rat spleen tissue, pig spleen tissue
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28359-1-AP targets UNC93B1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat, pig
免疫原 UNC93B1 fusion protein Ag27937 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 unc-93 homolog B1 (C. elegans)
别名 UNC93, Unc 93B1, unc 93 homolog B1 (C. elegans), Protein unc-93 homolog B1, Protein unc 93 homolog B1
计算分子量 597 aa, 67 kDa
观测分子量~70 kDa
GenBank蛋白编号BC101568
基因名称 UNC93B1
Gene ID (NCBI) 81622
RRIDAB_3086046
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9H1C4
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Unc-93 homolog B1 (UNC93B1) is a key regulator of nucleic acid (NA)-sensing Toll-like receptors (TLRs). UNC93B1 plays an important role in innate and adaptive immunity by regulating nucleotide-sensing Toll-like receptor (TLR) signaling. It's a transmembrane protein that is known to control the movement of TLRs from the endoplasmic reticulum-where TLRs are assembled-to endosomes. The calculated MW of UNC93B1 is 66 kDa, 28359-1-AP can detect a band around 70 kDa.

实验方案

Product Specific Protocols
WB protocol for UNC93B1 antibody 28359-1-APDownload protocol
IHC protocol for UNC93B1 antibody 28359-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols