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UNG Monoclonal antibody

UNG Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2B3B2

Cat no : 67490-1-Ig

Print datasheet

Synonyms

DGU, DKFZp781L1143, HIGM4, UDG, UNG, UNG1, UNG15, UNG2, uracil DNA glycosylase

验证数据展示

  • WB analysis using 67490-1-Ig

    WB analysis using 67490-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67490-1-Ig (UNG antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis of HepG2 using 67490-1-Ig

    WB analysis of HepG2 using 67490-1-Ig

    WB result of UNG antibody (67490-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-UNG transfected HepG2 cells.

  • IHC staining of human liver cancer using 67490-1-Ig

    IHC staining of human liver cancer using 67490-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67490-1-Ig (UNG antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with UNG antibody included

经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, LNCaP cells, HT-29 cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells
Positive IHC detected inhuman liver cancer tissue, human prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy UNG Ready-To-Use IHC Kit. UNG primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67490-1-Ig targets UNG in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity Human, mouse, rat
Immunogen UNG fusion protein Ag29979 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name uracil-DNA glycosylase
Synonyms DGU, DKFZp781L1143, HIGM4, UDG, UNG, UNG1, UNG15, UNG2, uracil DNA glycosylase
Calculated Molecular Weight 304 aa, 34 kDa
Observed Molecular Weight34 kDa
GenBank Accession NumberBC015205
Gene Symbol UNG
Gene ID (NCBI) 7374
RRIDAB_2918492
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP13051
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for UNG antibody 67490-1-IgDownload protocol
IHC protocol for UNG antibody 67490-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols