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验证数据展示

  • WB analysis using 31574-1-AP

    WB analysis using 31574-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31574-1-AP (UNK antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon using 31574-1-AP

    IHC staining of human colon using 31574-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 31574-1-AP (UNK antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HEK-293 using 31574-1-AP

    IF Staining of HEK-293 using 31574-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using UNK antibody (31574-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

UNK Polyclonal antibody

UNK Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 31574-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

ZC3HDC5, ZC3H5, unkempt homolog (Drosophila), KIAA1753

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293T cells, HeLa cells, Jurkat cells
Positive IF/ICC detected inHEK-293 cells, HeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31574-1-AP targets UNK in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 UNK fusion protein Ag36222 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 unkempt homolog (Drosophila)
别名 ZC3HDC5, ZC3H5, unkempt homolog (Drosophila), KIAA1753
观测分子量88~100 kDa
GenBank蛋白编号BC053362
基因名称 UNK
Gene ID (NCBI) 85451
RRIDAB_3670041
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ9C0B0
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Unkempt (UNK) is a sequence-specific RNA-binding protein which plays an important role in the establishment and maintenance of the early morphology of cortical neurons during embryonic development. UNK gene encodes a zinc finger/RING domain containing protein, as a negative regulator of the timing of photoreceptor differentiation.

实验方案

Product Specific Protocols
WB protocol for UNK antibody 31574-1-APDownload protocol
IF protocol for UNK antibody 31574-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols