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UPF1 Polyclonal antibody

UPF1 Polyclonal Antibody for WB, IF/ICC, IP, ELISA
Cat No. 23379-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human and More (1)

应用

WB, IF/ICC, IP, ELISA and More (2)

RENT1, Up-frameshift suppressor 1 homolog, Regulator of nonsense transcripts 1, KIAA0221, EC:3.6.4.12

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -


经过测试的应用

Positive WB detected inHeLa cells, Raji cells, SH-SY5Y cells
Positive IP detected inRaji cells
Positive IF/ICC detected inHepG2 cells, MCF-7 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

23379-1-AP targets UPF1 in WB, IF/ICC, IP, RIP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, IP, ELISA Application Description
文献引用应用WB, IF, RIP
经测试反应性 human
文献引用反应性human, mouse
免疫原 UPF1 fusion protein Ag16195 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 UPF1 regulator of nonsense transcripts homolog (yeast)
别名 RENT1, Up-frameshift suppressor 1 homolog, Regulator of nonsense transcripts 1, KIAA0221, EC:3.6.4.12
计算分子量123 kDa
观测分子量 123-130 kDa
GenBank蛋白编号BC039817
基因名称 UPF1
Gene ID (NCBI) 5976
RRIDAB_11232421
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ92900
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Up-Frameshift Suppressor 1 Homolog (UPF1) is the central factor in nonsense-mediated mRNA decay (NMD) and is also directly involved in telomere homeostasis, DNA replication, histone mRNA degradation and staufen-mediated mRNA decay (PMID: 29382845). It is a potential modulator of MALAT1 and that UPF1/MALAT1 pathway could be a therapeutic target for gastric cancer (PMID: 28942451). The molecular mass of UPF1 is 123-130 kDa.

实验方案

Product Specific Protocols
WB protocol for UPF1 antibody 23379-1-APDownload protocol
IF protocol for UPF1 antibody 23379-1-APDownload protocol
IP protocol for UPF1 antibody 23379-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Mol Cell

RAPIDASH: Tag-free enrichment of ribosome-associated proteins reveals composition dynamics in embryonic tissue, cancer cells, and macrophages

Authors - Teodorus Theo Susanto
humanWB

iScience

Mutational burden of XPNPEP3 leads to defects in mitochondrial complex I and cilia in NPHPL1

Authors - Lingxiao Tong
humanWB

Breast Cancer Res

Long noncoding RNA PVT1 promotes breast cancer proliferation and metastasis by binding miR-128-3p and UPF1.

Authors - Shuiyi Liu
humanWB

Bioengineered

The biological behavior of tRNA-derived fragment tRF-Leu-AAG in pancreatic cancer cells.

Authors - Shizhen Sui
humanWB

J Exp Clin Cancer Res

PTBP3 contributes to colorectal cancer growth and metastasis via translational activation of HIF-1α.

Authors - Pingfu Hou
humanWB

Cell Death Dis

PABPC1-induced stabilization of BDNF-AS inhibits malignant progression of glioblastoma cells through STAU1-mediated decay.

Authors - Rui Su
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