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验证数据展示

  • WB analysis using 28384-1-AP

    WB analysis using 28384-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28384-1-AP (UPF1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293 using 28384-1-AP

    WB analysis of HEK-293 using 28384-1-AP

    WB result of UPF1 antibody (28384-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-UPF1 transfected HEK-293 cells.

  • IHC staining of human breast cancer using 28384-1-AP

    IHC staining of human breast cancer using 28384-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 28384-1-AP (UPF1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human breast cancer using 28384-1-AP

    IHC staining of human breast cancer using 28384-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 28384-1-AP (UPF1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Featured Product
  • KD/KO Validated

UPF1 Polyclonal antibody

UPF1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 28384-1-AP

Print datasheet

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IHC, ELISA

Synonyms

ATP dependent helicase RENT1, FLJ43809, FLJ46894, HUPF1, KIAA0221, NORF1, pNORF1, RENT1, UPF1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, Raji cells, SH-SY5Y cells, SKOV-3 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28384-1-AP targets UPF1 in WB, IHC, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity Human
Immunogen UPF1 fusion protein Ag29057 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name UPF1 regulator of nonsense transcripts homolog (yeast)
Synonyms ATP dependent helicase RENT1, FLJ43809, FLJ46894, HUPF1, KIAA0221, NORF1, pNORF1, RENT1, UPF1
Calculated Molecular Weight123 kDa
Observed Molecular Weight 123-130 kDa
GenBank Accession NumberBC039817
Gene Symbol UPF1
Gene ID (NCBI) 5976
RRIDAB_2881130
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ92900
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Up-Frameshift Suppressor 1 Homolog (UPF1) is the central factor in nonsense-mediated mRNA decay (NMD) and is also directly involved in telomere homeostasis, DNA replication, histone mRNA degradation and staufen-mediated mRNA decay (PMID: 29382845). It is a potential modulator of MALAT1 and that UPF1/MALAT1 pathway could be a therapeutic target for gastric cancer (PMID: 28942451). The molecular mass of UPF1 is 123-130 kDa.

实验方案

Product Specific Protocols
WB protocol for UPF1 antibody 28384-1-APDownload protocol
IHC protocol for UPF1 antibody 28384-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols