验证数据展示
经过测试的应用
Positive WB detected in | HEK-293 cells, HeLa cells, Raji cells, SH-SY5Y cells, SKOV-3 cells |
Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:500-1:2000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
28384-1-AP targets UPF1 in WB, IHC, ELISA applications and shows reactivity with Human samples.
经测试应用 | WB, IHC, ELISA Application Description |
经测试反应性 | Human |
免疫原 | UPF1 fusion protein Ag29057 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | UPF1 regulator of nonsense transcripts homolog (yeast) |
别名 | ATP dependent helicase RENT1, FLJ43809, FLJ46894, HUPF1, KIAA0221, NORF1, pNORF1, RENT1, UPF1 |
计算分子量 | 123 kDa |
观测分子量 | 123-130 kDa |
GenBank蛋白编号 | BC039817 |
基因名称 | UPF1 |
Gene ID (NCBI) | 5976 |
RRID | AB_2881130 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | Q92900 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Up-Frameshift Suppressor 1 Homolog (UPF1) is the central factor in nonsense-mediated mRNA decay (NMD) and is also directly involved in telomere homeostasis, DNA replication, histone mRNA degradation and staufen-mediated mRNA decay (PMID: 29382845). It is a potential modulator of MALAT1 and that UPF1/MALAT1 pathway could be a therapeutic target for gastric cancer (PMID: 28942451). The molecular mass of UPF1 is 123-130 kDa.
实验方案
Product Specific Protocols | |
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WB protocol for UPF1 antibody 28384-1-AP | Download protocol |
IHC protocol for UPF1 antibody 28384-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |