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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 66898-1-Ig (same clone as 66898-1-PBS)

    WB analysis using 66898-1-Ig (same clone as 66898-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66898-1-Ig (UPF1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66898-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 66898-1-Ig (same clone as 66898-1-PBS)

    IHC staining of human breast cancer using 66898-1-Ig (same clone as 66898-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66898-1-Ig (UPF1 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66898-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 66898-1-Ig (same clone as 66898-1-PBS)

    IHC staining of human breast cancer using 66898-1-Ig (same clone as 66898-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66898-1-Ig (UPF1 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66898-1-PBS in a different storage buffer formulation.

  • WB analysis using 66898-1-Ig (same clone as 66898-1-PBS)

    WB analysis using 66898-1-Ig (same clone as 66898-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66898-1-Ig (UPF1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66898-1-PBS in a different storage buffer formulation.

  • WB analysis of HEK-293 using 66898-1-Ig (same clone as 66898-1-PBS)

    WB analysis of HEK-293 using 66898-1-Ig (same clone as 66898-1-PBS)

    WB result of UPF1 antibody (66898-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-UPF1 transfected HEK-293 cells. This data was developed using the same antibody clone with 66898-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 66898-1-Ig (same clone as 66898-1-PBS)

    IF Staining of HepG2 using 66898-1-Ig (same clone as 66898-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using UPF1 antibody (66898-1-Ig, Clone: 3B6B5 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66898-1-PBS in a different storage buffer formulation.

  • FC experiment of HepG2 using 66898-1-Ig (same clone as 66898-1-PBS)

    FC experiment of HepG2 using 66898-1-Ig (same clone as 66898-1-PBS)

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human UPF1 (66898-1-Ig, Clone:3B6B5) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 66898-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

UPF1 Monoclonal antibody, PBS Only

UPF1 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 66898-1-PBS

Print datasheet

CloneNo. 3B6B5

Host / Isotype

Mouse / IgG1

Reactivity

rat, mouse, human

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Synonyms

RENT1, Regulator of nonsense transcripts 1, KIAA0221, EC:3.6.4.12, ATP-dependent helicase RENT1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size: 

-/ -

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产品信息

66898-1-PBS targets UPF1 in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with rat, mouse, human samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), Indirect ELISA Application Description
Tested Reactivity rat, mouse, human
Immunogen UPF1 fusion protein Ag28320 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name UPF1 regulator of nonsense transcripts homolog (yeast)
Synonyms RENT1, Regulator of nonsense transcripts 1, KIAA0221, EC:3.6.4.12, ATP-dependent helicase RENT1
Calculated Molecular Weight123 kDa
Observed Molecular Weight 123-130 kDa
GenBank Accession NumberBC039817
Gene Symbol UPF1
Gene ID (NCBI) 5976
RRIDAB_2882227
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ92900
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Up-Frameshift Suppressor 1 Homolog (UPF1) is the central factor in nonsense-mediated mRNA decay (NMD) and is also directly involved in telomere homeostasis, DNA replication, histone mRNA degradation and staufen-mediated mRNA decay (PMID: 29382845). It is a potential modulator of MALAT1 and that UPF1/MALAT1 pathway could be a therapeutic target for gastric cancer (PMID: 28942451). The molecular mass of UPF1 is 123-130 kDa.