验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, Jurkat cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
55022-1-AP targets UPF2 in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 | Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | UPF2 regulator of nonsense transcripts homolog (yeast) |
| 别名 | HUPF2, KIAA1408, RENT2, smg 3, UPF2 |
| 计算分子量 | 148 kDa |
| 观测分子量 | 148 kDa |
| GenBank蛋白编号 | NM_015542 |
| 基因名称 | UPF2 |
| Gene ID (NCBI) | 26019 |
| RRID | AB_3086431 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9HAU5 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
UPF2 is involved in nonsense-mediated decay (NMD) of mRNAs containing premature stop codons by associating with the nuclear exon junction complex (EJC). UPF2 also plays an important role in development and cell differentiation.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for UPF2 antibody 55022-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |