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UPF2 Polyclonal antibody

UPF2 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 55022-1-AP

Print datasheet

Synonyms

HUPF2, KIAA1408, RENT2, smg 3, UPF2

验证数据展示

  • WB analysis using 55022-1-AP

    WB analysis using 55022-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 55022-1-AP (UPF2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inHeLa cells, Jurkat cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

55022-1-AP targets UPF2 in WB, ELISA applications and shows reactivity with human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name UPF2 regulator of nonsense transcripts homolog (yeast)
Synonyms HUPF2, KIAA1408, RENT2, smg 3, UPF2
Calculated Molecular Weight 148 kDa
Observed Molecular Weight148 kDa
GenBank Accession NumberNM_015542
Gene Symbol UPF2
Gene ID (NCBI) 26019
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9HAU5
Storage Buffer 0.1M NaHCO3, 0.1M glycine, 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

UPF2 is involved in nonsense-mediated decay (NMD) of mRNAs containing premature stop codons by associating with the nuclear exon junction complex (EJC). UPF2 also plays an important role in development and cell differentiation.

实验方案

Product Specific Protocols
WB protocol for UPF2 antibody 55022-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols