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UPF3B Polyclonal antibody

UPF3B Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 23301-1-AP

Print datasheet

Synonyms

RENT3B, UPF3X, MRXS14, hUpf3p-X, hUpf3p X

验证数据展示

  • WB analysis using 23301-1-AP

    WB analysis using 23301-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 23301-1-AP (UPF3B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary tumor using 23301-1-AP

    IHC staining of human ovary tumor using 23301-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 23301-1-AP (UPF3B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human ovary tumor using 23301-1-AP

    IHC staining of human ovary tumor using 23301-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 23301-1-AP (UPF3B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inHEK-293 cells, mouse brain tissue, K-562 cells, Jurkat cells, HeLa cells
Positive IHC detected inhuman ovary tumor tissue, human skeletal muscle tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IHCSee 1 publications below

产品信息

23301-1-AP targets UPF3B in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsIHC
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen UPF3B fusion protein Ag18568 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name UPF3 regulator of nonsense transcripts homolog B (yeast)
Synonyms RENT3B, UPF3X, MRXS14, hUpf3p-X, hUpf3p X
Calculated Molecular Weight 483 aa, 58 kDa
Observed Molecular Weight 58 kDa
GenBank Accession NumberBC121017
Gene Symbol UPF3B
Gene ID (NCBI) 65109
RRIDAB_2879249
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BZI7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for UPF3B antibody 23301-1-APDownload protocol
IHC protocol for UPF3B antibody 23301-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

Front Genet

Molecular Characterization and Clinical Relevance of RNA Binding Proteins in Colorectal Cancer.

Authors - Zhen Zhang
View Article