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UPP1 Polyclonal antibody

UPP1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 14186-1-AP

Print datasheet

Synonyms

UDRPASE, UP, UPASE, UPase 1, UPP, UPP1, UrdPase 1, uridine phosphorylase 1

验证数据展示

  • WB analysis of U2OS using 14186-1-AP

    WB analysis of U2OS using 14186-1-AP

    U2OS cells were subjected to SDS PAGE followed by western blot with 14186-1-AP (UPP1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse small intestine using 14186-1-AP

    IHC staining of mouse small intestine using 14186-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 14186-1-AP (UPP1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with UPP1 antibody included

经过测试的应用

Positive WB detected inU2OS cells, SGC-7901 cells
Positive IHC detected inmouse small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy UPP1 Ready-To-Use IHC Kit. UPP1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14186-1-AP targets UPP1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB, IHC, IF
Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Immunogen UPP1 fusion protein Ag5441 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name uridine phosphorylase 1
Synonyms UDRPASE, UP, UPASE, UPase 1, UPP, UPP1, UrdPase 1, uridine phosphorylase 1
Calculated Molecular Weight 34 kDa
Observed Molecular Weight 34 kDa
GenBank Accession NumberBC047030
Gene Symbol UPP1
Gene ID (NCBI) 7378
RRIDAB_2878025
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ16831
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for UPP1 antibody 14186-1-APDownload protocol
IHC protocol for UPP1 antibody 14186-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB,IHC

Arthritis Rheumatol

SHP2 inhibition attenuates osteoarthritis by maintaining homeostasis of cartilage metabolism via the DOK1/UPP1/uridine cascade.

Authors - Qianqian Liu
View Article
human,mouseWB,IHC,IF

Nat Commun

UPP1 promotes lung adenocarcinoma progression through the induction of an immunosuppressive microenvironment

Authors - Yin Li
View Article
humanWB

Cancer Cell Int

p53 activation enhances the sensitivity of non-small cell lung cancer to the combination of SH003 and docetaxel by inhibiting de novo pyrimidine synthesis

Authors - Yu-Jeong Choi
View Article