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验证数据展示

  • WB analysis using 28114-1-AP

    WB analysis using 28114-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28114-1-AP (UQCC antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of human intrahepatic cholangiocarcinoma using 28114-1-AP

    IHC staining of human intrahepatic cholangiocarcinoma using 28114-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 28114-1-AP (UQCC antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human intrahepatic cholangiocarcinoma using 28114-1-AP

    IHC staining of human intrahepatic cholangiocarcinoma using 28114-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 28114-1-AP (UQCC antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

UQCC Polyclonal antibody

UQCC Polyclonal Antibody for WB, IHC, ELISA
Cat No. 28114-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

CBP3, C20orf44, BZFB, bFZb, bFGF-repressed Zic-binding protein

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, K-562 cells, MCF-7 cells, LNCaP cells
Positive IHC detected inhuman intrahepatic cholangiocarcinoma tissue, human ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28114-1-AP targets UQCC in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原 UQCC fusion protein Ag27123 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ubiquinol-cytochrome c reductase complex chaperone
别名 CBP3, C20orf44, BZFB, bFZb, bFGF-repressed Zic-binding protein
计算分子量 299 aa, 35 kDa
观测分子量 21-27 kDa
GenBank蛋白编号BC105791
基因名称 UQCC
Gene ID (NCBI) 55245
RRIDAB_2881065
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NVA1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

UQCC is a transmembrane protein that is structurally similar to the mouse basic fibroblast growth factor repressed ZIC-binding protein. In mouse this protein may be involved in fibroblast growth factor regulated growth control. In humans, polymorphisms in this gene are associated with variation in human height and osteoarthritis. Alternate splicing results in multiple transcript variant.

实验方案

Product Specific Protocols
WB protocol for UQCC antibody 28114-1-APDownload protocol
IHC protocol for UQCC antibody 28114-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols