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验证数据展示

  • WB analysis using 28114-1-AP

    WB analysis using 28114-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28114-1-AP (UQCC antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of human intrahepatic cholangiocarcinoma using 28114-1-AP

    IHC staining of human intrahepatic cholangiocarcinoma using 28114-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 28114-1-AP (UQCC antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human intrahepatic cholangiocarcinoma using 28114-1-AP

    IHC staining of human intrahepatic cholangiocarcinoma using 28114-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 28114-1-AP (UQCC antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

UQCC Polyclonal antibody

UQCC Polyclonal Antibody for WB, IHC, ELISA
Cat No. 28114-1-AP

Print datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

CBP3, C20orf44, BZFB, bFZb, bFGF-repressed Zic-binding protein

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, K-562 cells, MCF-7 cells, LNCaP cells
Positive IHC detected inhuman intrahepatic cholangiocarcinoma tissue, human ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28114-1-AP targets UQCC in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human
Immunogen UQCC fusion protein Ag27123 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ubiquinol-cytochrome c reductase complex chaperone
Synonyms CBP3, C20orf44, BZFB, bFZb, bFGF-repressed Zic-binding protein
Calculated Molecular Weight 299 aa, 35 kDa
Observed Molecular Weight 21-27 kDa
GenBank Accession NumberBC105791
Gene Symbol UQCC
Gene ID (NCBI) 55245
RRIDAB_2881065
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NVA1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

UQCC is a transmembrane protein that is structurally similar to the mouse basic fibroblast growth factor repressed ZIC-binding protein. In mouse this protein may be involved in fibroblast growth factor regulated growth control. In humans, polymorphisms in this gene are associated with variation in human height and osteoarthritis. Alternate splicing results in multiple transcript variant.

实验方案

Product Specific Protocols
WB protocol for UQCC antibody 28114-1-APDownload protocol
IHC protocol for UQCC antibody 28114-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols