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  • Featured Product
  • KD/KO Validated

UQCRQ Polyclonal antibody

UQCRQ Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 14975-1-AP

Print datasheet

Synonyms

QPC, QP C, QCR8, Cytochrome b-c1 complex subunit 8, Complex III subunit VIII

验证数据展示

  • WB analysis using 14975-1-AP

    WB analysis using 14975-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 14975-1-AP (UQCRQ antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IP experiment of HepG2 using 14975-1-AP

    IP experiment of HepG2 using 14975-1-AP

    IP result of anti-UQCRQ (IP:14975-1-AP, 4ug; Detection:14975-1-AP 1:1000) with HepG2 cells lysate 3360 ug.

  • IHC staining of human liver cancer using 14975-1-AP

    IHC staining of human liver cancer using 14975-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14975-1-AP (UQCRQ antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with UQCRQ antibody included
  • IHC staining of human liver cancer using 14975-1-AP

    IHC staining of human liver cancer using 14975-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14975-1-AP (UQCRQ antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with UQCRQ antibody included
  • IF Staining of HepG2 using 14975-1-AP

    IF Staining of HepG2 using 14975-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using UQCRQ antibody (14975-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHepG2 cells, human liver tissue
Positive IP detected inHepG2 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy UQCRQ Ready-To-Use IHC Kit. UQCRQ primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14975-1-AP targets UQCRQ in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, IP, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Immunogen UQCRQ fusion protein Ag6886 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ubiquinol-cytochrome c reductase, complex III subunit VII, 9.5kDa
Synonyms QPC, QP C, QCR8, Cytochrome b-c1 complex subunit 8, Complex III subunit VIII
Calculated Molecular Weight 10 kDa
Observed Molecular Weight 10 kDa
GenBank Accession NumberBC001390
Gene Symbol UQCRQ
Gene ID (NCBI) 27089
RRIDAB_2288356
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO14949
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

UQCRQ(Cytochrome b-c1 complex subunit 8) belongs to the UQCRQ/QCR8 family. UQCRQ is a ubiquinone-binding protein localized to the cytochrome bc1 region of the mitochondrial respiratory chain. The deduced 110-amino acid protein has a relatively hydrophobic and basic N-terminal region, an aspartic acid-rich middle region, and a glutamic acid- and lysine-rich C-terminal region.

实验方案

Product Specific Protocols
WB protocol for UQCRQ antibody 14975-1-APDownload protocol
IHC protocol for UQCRQ antibody 14975-1-APDownload protocol
IF protocol for UQCRQ antibody 14975-1-APDownload protocol
IP protocol for UQCRQ antibody 14975-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Int J Mol Sci

Deficiency of T-Cell Intracellular Antigen 1 in Murine Embryonic Fibroblasts Is Associated with Changes in Mitochondrial Morphology and Respiration.

Authors - Isabel Carrascoso
View Article
humanWB

Stem Cells

The Transcription Factor 7-Like 2-Peroxisome Proliferator-Activated Receptor Gamma Coactivator-1 Alpha Axis Connects Mitochondrial Biogenesis and Metabolic Shift with Stem Cell Commitment to Hepatic Differentiation.

Authors - Anaïs Wanet
View Article
ratWB

Cell Death Dis

Calcium sensing receptor protects high glucose-induced energy metabolism disorder via blocking gp78-ubiquitin proteasome pathway.

Authors - Yuehong Wang
View Article
human

J Biol Chem

Mitochondrial reactive oxygen species regulate transforming growth factor-β signaling.

Authors - Jain Manu M
  • KD Validated
View Article
mouseWB

Am J Physiol Endocrinol Metab

Exogenous H2S reduces acetylation levels of mitochondrial respiratory enzymes via regulating NAD+-SIRT3 pathway in cardiac tissues of db/db mice.

Authors - Yu Sun
View Article
mouseWB

Front Endocrinol (Lausanne)

The Impaired Bioenergetics of Diabetic Cardiac Microvascular Endothelial Cells.

Authors - Haitao Zhang
View Article