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验证数据展示

  • WB analysis using 15285-1-AP

    WB analysis using 15285-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15285-1-AP (URM1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human hepatocirrhosis using 15285-1-AP

    IHC staining of human hepatocirrhosis using 15285-1-AP

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 15285-1-AP (URM1 antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of mouse liver using 15285-1-AP

    IHC staining of mouse liver using 15285-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 15285-1-AP (URM1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse liver using 15285-1-AP

    IHC staining of mouse liver using 15285-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 15285-1-AP (URM1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of human placenta using 15285-1-AP

    WB analysis of human placenta using 15285-1-AP

    human placenta tissue were subjected to SDS PAGE followed by western blot with 15285-1-AP (URM1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human hepatocirrhosis using 15285-1-AP

    IHC staining of human hepatocirrhosis using 15285-1-AP

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 15285-1-AP (URM1 antibody) at dilution of 1:100 (under 40x lens).

  • IP experiment of HepG2 using 15285-1-AP

    IP experiment of HepG2 using 15285-1-AP

    IP result of anti-URM1 (IP:15285-1-AP, 4ug; Detection:15285-1-AP 1:500) with HepG2 cells lysate 3360 ug.

  • Featured Product
  • KD/KO验证

URM1 Polyclonal antibody

URM1 Polyclonal Antibody for WB, IP, IHC, ELISA
Cat No. 15285-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IP, IHC, ELISA

别名

C9orf74, URM1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA549 cells, HepG2 cells, mouse liver tissue, human placenta tissue
Positive IP detected inHepG2 cells
Positive IHC detected inmouse liver tissue, human hepatocirrhosis tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15285-1-AP targets URM1 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IP, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原 URM1 fusion protein Ag7448 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ubiquitin related modifier 1 homolog (S. cerevisiae)
别名 C9orf74, URM1
计算分子量 11 kDa
观测分子量 11 kDa
GenBank蛋白编号BC003581
基因名称 URM1
Gene ID (NCBI) 81605
RRIDAB_2213808
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9BTM9
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for URM1 antibody 15285-1-APDownload protocol
IHC protocol for URM1 antibody 15285-1-APDownload protocol
IP protocol for URM1 antibody 15285-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Mol Cell Biol

Human AlkB homolog ABH8 Is a tRNA methyltransferase required for wobble uridine modification and DNA damage survival.

Authors - Fu Dragony D
  • KD Validated
View Article
humanWB

Hum Mol Genet

Cytosolic HSC20 integrates de novo iron-sulfur cluster biogenesis with the CIAO1-mediated transfer to recipients.

Authors - Ki Soon Kim
View Article