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  • Featured Product
  • KD/KO Validated

URM1 Polyclonal antibody

URM1 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 15285-1-AP

Print datasheet

Synonyms

C9orf74, URM1

验证数据展示

  • WB analysis using 15285-1-AP

    WB analysis using 15285-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15285-1-AP (URM1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human hepatocirrhosis using 15285-1-AP

    IHC staining of human hepatocirrhosis using 15285-1-AP

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 15285-1-AP (URM1 antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of mouse liver using 15285-1-AP

    IHC staining of mouse liver using 15285-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 15285-1-AP (URM1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse liver using 15285-1-AP

    IHC staining of mouse liver using 15285-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 15285-1-AP (URM1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of human placenta using 15285-1-AP

    WB analysis of human placenta using 15285-1-AP

    human placenta tissue were subjected to SDS PAGE followed by western blot with 15285-1-AP (URM1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human hepatocirrhosis using 15285-1-AP

    IHC staining of human hepatocirrhosis using 15285-1-AP

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 15285-1-AP (URM1 antibody) at dilution of 1:100 (under 40x lens).

  • IP experiment of HepG2 using 15285-1-AP

    IP experiment of HepG2 using 15285-1-AP

    IP result of anti-URM1 (IP:15285-1-AP, 4ug; Detection:15285-1-AP 1:500) with HepG2 cells lysate 3360 ug.

经过测试的应用

Positive WB detected inA549 cells, HepG2 cells, mouse liver tissue, human placenta tissue
Positive IP detected inHepG2 cells
Positive IHC detected inmouse liver tissue, human hepatocirrhosis tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15285-1-AP targets URM1 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IP, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen URM1 fusion protein Ag7448 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ubiquitin related modifier 1 homolog (S. cerevisiae)
Synonyms C9orf74, URM1
Calculated Molecular Weight 11 kDa
Observed Molecular Weight 11 kDa
GenBank Accession NumberBC003581
Gene Symbol URM1
Gene ID (NCBI) 81605
RRIDAB_2213808
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BTM9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for URM1 antibody 15285-1-APDownload protocol
IHC protocol for URM1 antibody 15285-1-APDownload protocol
IP protocol for URM1 antibody 15285-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Mol Cell Biol

Human AlkB homolog ABH8 Is a tRNA methyltransferase required for wobble uridine modification and DNA damage survival.

Authors - Fu Dragony D
  • KD Validated
View Article
humanWB

Hum Mol Genet

Cytosolic HSC20 integrates de novo iron-sulfur cluster biogenesis with the CIAO1-mediated transfer to recipients.

Authors - Ki Soon Kim
View Article