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UROD Polyclonal antibody

UROD Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 15547-1-AP

Print datasheet

Synonyms

URO-D, EC:4.1.1.37

验证数据展示

  • WB analysis using 15547-1-AP

    WB analysis using 15547-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15547-1-AP (UROD antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IF Staining of HEK-293 using 15547-1-AP

    IF Staining of HEK-293 using 15547-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using UROD antibody (15547-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • IHC staining of human kidney using 15547-1-AP

    IHC staining of human kidney using 15547-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15547-1-AP (UROD antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human kidney using 15547-1-AP

    IHC staining of human kidney using 15547-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15547-1-AP (UROD antibody) at dilution of 1:100 (under 40x lens).

  • IP experiment of K-562 using 15547-1-AP

    IP experiment of K-562 using 15547-1-AP

    IP result of anti-UROD (IP:15547-1-AP, 4ug; Detection:15547-1-AP 1:500) with K-562 cells lysate 4400 ug.

经过测试的应用

Positive WB detected inK-562 cells, HepG2 cells, HEK-293 cells, rat kidney tissue, mouse liver tissue, rat liver tissue
Positive IP detected inK-562 cells
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHEK-293 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

15547-1-AP targets UROD in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, IP, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen UROD fusion protein Ag7905 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name uroporphyrinogen decarboxylase
Synonyms URO-D, EC:4.1.1.37
Calculated Molecular Weight 41 kDa
Observed Molecular Weight 41 kDa
GenBank Accession NumberBC001778
Gene Symbol UROD
Gene ID (NCBI) 7389
RRIDAB_2213813
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP06132
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for UROD antibody 15547-1-APDownload protocol
IHC protocol for UROD antibody 15547-1-APDownload protocol
IF protocol for UROD antibody 15547-1-APDownload protocol
IP protocol for UROD antibody 15547-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Mol Cell

Metabolic-scale gene activation screens identify SLCO2B1 as a heme transporter that enhances cellular iron availability.

Authors - Gokhan Unlu
View Article