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验证数据展示

  • WB analysis of HEK-293 using 25218-1-AP

    WB analysis of HEK-293 using 25218-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 25218-1-AP (USE1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human small intestine using 25218-1-AP

    IHC staining of human small intestine using 25218-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 25218-1-AP (USE1 Antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human small intestine using 25218-1-AP

    IHC staining of human small intestine using 25218-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 25218-1-AP (USE1 Antibody) at dilution of 1:200 (under 40x lens).

  • Featured Product
  • KD/KO验证

USE1 Polyclonal antibody

USE1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 25218-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

MDS032, P31, SLT1, USE1, USE1 like protein, USE1L, Vesicle transport protein USE1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells, Jurkat cells
Positive IHC detected inhuman small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:100-1:400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25218-1-AP targets USE1 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human
文献引用反应性human
免疫原 USE1 fusion protein Ag18587 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 unconventional SNARE in the ER 1 homolog (S. cerevisiae)
别名 MDS032, P31, SLT1, USE1, USE1 like protein, USE1L, Vesicle transport protein USE1
计算分子量 259 aa, 29 kDa
观测分子量 29 kDa
GenBank蛋白编号BC008455
基因名称 USE1
Gene ID (NCBI) 55850
RRIDAB_2879966
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NZ43
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

USE1(Vesicle transport protein USE1) is also named as USE1L, p31 and belongs to the USE1 family. It may be an essential molecule involved in the maintenance of ER morphology (and its deficiency leads to ER stress-induced apoptosis). USE1 also participates in the degradative function of lysosomes. This protein has 3 isoforms produced by alternative splicing.

实验方案

Product Specific Protocols
WB protocol for USE1 antibody 25218-1-APDownload protocol
IHC protocol for USE1 antibody 25218-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

PLoS Pathog

SNARE protein USE1 is involved in the glycosylation and the expression of mumps virus fusion protein and important for viral propagation

Authors - Yaqing Liu
  • KD Validated
View Article
humanWB

J Cell Sci

A trap mutant reveals the physiological client spectrum of TRC40.

Authors - Javier Coy-Vergara
View Article