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验证数据展示

  • WB analysis using 22327-1-AP

    WB analysis using 22327-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 22327-1-AP (USF1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 22327-1-AP

    IF Staining of HeLa using 22327-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 22327-1-AP (USF1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

  • IHC staining of human stomach cancer using 22327-1-AP

    IHC staining of human stomach cancer using 22327-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 22327-1-AP (USF1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with USF1 antibody included
  • IHC staining of human stomach cancer using 22327-1-AP

    IHC staining of human stomach cancer using 22327-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 22327-1-AP (USF1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with USF1 antibody included
  • IHC staining of human stomach cancer using 22327-1-AP

    IHC staining of human stomach cancer using 22327-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 22327-1-AP (USF1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with USF1 antibody included
  • Featured Product
  • KD/KO验证

USF1 Polyclonal antibody

USF1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 22327-1-AP

产品说明书

引用文献(3)

宿主/亚型

Rabbit / IgG

种属反应性

human and More (1)

应用

WB, IHC, IF/ICC, ELISA

别名

bHLHb11, FCHL, FCHL1, HYPLIP1, MLTF, MLTFI, UEF, Upstream stimulatory factor 1, USF, USF1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, K-562 cells, Jurkat cells
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy USF1 Ready-To-Use IHC Kit. USF1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

22327-1-AP targets USF1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB
经测试反应性 human
文献引用反应性human, mouse
免疫原 USF1 fusion protein Ag17789 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 upstream transcription factor 1
别名 bHLHb11, FCHL, FCHL1, HYPLIP1, MLTF, MLTFI, UEF, Upstream stimulatory factor 1, USF, USF1
计算分子量 34 kDa
观测分子量 43 kDa
GenBank蛋白编号BC035505
基因名称 USF1
Gene ID (NCBI) 7391
RRIDAB_2879073
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen Affinity purified
UNIPROT IDP22415
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Upstream stimulatory factor 1 (USF1),also name Class B basic helix-loop-helix protein 11. it is a major late transcription factor 1.USF1 is a member of the basic helix-loop-helix leucine zipper family, and can function as a cellular transcription factor. The encoded protein can activate transcription through pyrimidine-rich initiator (Inr) elements and E box motifs. USF1 has been linked to familial combined hyperlipidemia (FCHL). The USF1 exists two isoform and the calculated molecular weight of USF1 is a 32 kDa and 27 kDa. USF1 has acetylated form and phosphorylated form, so the molecular weight of USF1 can migrate to about 40 kDa

实验方案

Product Specific Protocols
WB protocol for USF1 antibody 22327-1-APDownload protocol
IHC protocol for USF1 antibody 22327-1-APDownload protocol
IF protocol for USF1 antibody 22327-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Genome Biol

Acute depletion of CTCF rewires genome-wide chromatin accessibility.

Authors - Beisi Xu
  • KO Validated
View Article
humanWB

Elife

Functional interrogation of HOXA9 regulome in MLLr leukemia via reporter-based CRISPR/Cas9 screen.

Authors - Hao Zhang
  • KO Validated
View Article
human,mouseWB

J Oral Pathol Med

LncRNA LINC00152 promotes oral squamous cell carcinoma growth via enhancing Upstream Transcription Factor 1 mediated Mitochondrial Ribosomal Protein L52 transcription.

Authors - Xiuhe Zou
  • KD Validated
View Article