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验证数据展示

  • WB analysis of A549 using 17226-1-AP

    WB analysis of A549 using 17226-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 17226-1-AP (USHBP1 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

  • IHC staining of human heart using 17226-1-AP

    IHC staining of human heart using 17226-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 17226-1-AP (USHBP1 Antibody) at dilution of 1:50 (under 40x lens).

  • IHC staining of human small intestine using 17226-1-AP

    IHC staining of human small intestine using 17226-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 17226-1-AP (USHBP1 Antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of A549 using 17226-1-AP

    IF Staining of A549 using 17226-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed A549 cells using 17226-1-AP (USHBP1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

USHBP1 Polyclonal antibody

USHBP1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 17226-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, ELISA

别名

MCC2, MCC 2, Harmonin-binding protein USHBP1, AIEBP, AIE-75-binding protein

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA549 cells, COLO 320 cells, mouse heart tissue
Positive IHC detected inhuman heart tissue, human small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA549 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17226-1-AP targets USHBP1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原 USHBP1 fusion protein Ag11064 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 Usher syndrome 1C binding protein 1
别名 MCC2, MCC 2, Harmonin-binding protein USHBP1, AIEBP, AIE-75-binding protein
计算分子量 703 aa, 76 kDa
观测分子量 90-100 kDa
GenBank蛋白编号BC027910
基因名称 USHBP1
Gene ID (NCBI) 83878
RRIDAB_2878364
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8N6Y0
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

USHBP1, also named as Mutated in colon cancer protein 2 or AIE-75-binding protein, is a 703 amino acid protein, which belongs to the MCC family. Highest level of expression is in heart, and moderate to low expression in skeletal muscle, kidney, liver, small intestine, placenta and lung.

实验方案

Product Specific Protocols
WB protocol for USHBP1 antibody 17226-1-APDownload protocol
IHC protocol for USHBP1 antibody 17226-1-APDownload protocol
IF protocol for USHBP1 antibody 17226-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols