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USHBP1 Polyclonal antibody

USHBP1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 17226-1-AP

Print datasheet

Synonyms

MCC2, MCC 2, Harmonin-binding protein USHBP1, AIEBP, AIE-75-binding protein

验证数据展示

  • WB analysis of A549 using 17226-1-AP

    WB analysis of A549 using 17226-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 17226-1-AP (USHBP1 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

  • IHC staining of human heart using 17226-1-AP

    IHC staining of human heart using 17226-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 17226-1-AP (USHBP1 Antibody) at dilution of 1:50 (under 40x lens).

  • IHC staining of human small intestine using 17226-1-AP

    IHC staining of human small intestine using 17226-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 17226-1-AP (USHBP1 Antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of A549 using 17226-1-AP

    IF Staining of A549 using 17226-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed A549 cells using 17226-1-AP (USHBP1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inA549 cells, COLO 320 cells, mouse heart tissue
Positive IHC detected inhuman heart tissue, human small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA549 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17226-1-AP targets USHBP1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen USHBP1 fusion protein Ag11064 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name Usher syndrome 1C binding protein 1
Synonyms MCC2, MCC 2, Harmonin-binding protein USHBP1, AIEBP, AIE-75-binding protein
Calculated Molecular Weight 703 aa, 76 kDa
Observed Molecular Weight 90-100 kDa
GenBank Accession NumberBC027910
Gene Symbol USHBP1
Gene ID (NCBI) 83878
RRIDAB_2878364
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8N6Y0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

USHBP1, also named as Mutated in colon cancer protein 2 or AIE-75-binding protein, is a 703 amino acid protein, which belongs to the MCC family. Highest level of expression is in heart, and moderate to low expression in skeletal muscle, kidney, liver, small intestine, placenta and lung.

实验方案

Product Specific Protocols
WB protocol for USHBP1 antibody 17226-1-APDownload protocol
IHC protocol for USHBP1 antibody 17226-1-APDownload protocol
IF protocol for USHBP1 antibody 17226-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols